Magnesium fractions in serum of healthy individuals and capd patients, measured by an ion-selective electrode and ultrafiltration
| Autor: | Gerard T. Sanders, Henk J. Huijgen, Huub E. van Ingen, Wim Th. Kok |
|---|---|
| Přispěvatelé: | HIMS former research (FNWI) |
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male Clinical Biochemistry Carbonates Ultrafiltration chemistry.chemical_element Ionized magnesium Phosphates law.invention Ion selective electrode Peritoneal Dialysis Continuous Ambulatory Reference Values law Humans Magnesium Citrates Aged Chromatography Continuous ambulatory peritoneal dialysis General Medicine Middle Aged Chromatography Ion Exchange chemistry Evaluation Studies as Topic Reference values Healthy individuals Kidney Failure Chronic Female Atomic absorption spectroscopy Ion-Selective Electrodes |
| Zdroj: | Clinical biochemistry, 29, 261-266. Elsevier |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/0009-9120(96)84729-b |
| Popis: | Objectives: Validation and comparison of a magnesium ionselective electrode (ISE) with a cation-exchange resin technique, followed by determination of all magnesium fractions in serum of healthy volunteers and continuous ambulatory peritoneal dialysis (CAPD) patients. Design and Methods: The analytical aspect has been studied by measuring the influence of complexing agents on the fraction ionized magnesium (friMg 2+ ). A theoretical approximation of friMg 2+ , based on mass equilibria and complexation constants, was calculated and compared with the measurements. Results: ISE measurements showed good agreement with theory. Reference values of the ionized, protein-bound, and complexed magnesium fractions were (mean ± SD) 0.65 ± 0.04, 0.27 ± 0.04, and 0.08 ± 0.03, respectively. Fractions obtained in the CAPD group were 0.62 ± 0.04, 0.22 ± 0.05, and 0.16 ± 0.05, respectively, and differed significantly from the values of the reference population. Conclusions: All known serum magnesium parameters can be established by a combination of ultrafiltration, atomic absorption spectrometry, and ISE measurements. Unknown complexing compounds most probably account for the increased fraction of complexed magnesium in the serum of CAPD patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect