Simultaneous single-cell phenotype analysis of hepatocellular carcinoma CTCs using a SERS-aptamer based microfluidic chip
| Autor: | Changbiao Zhan, Yang Lu, Rongke Gao, Baoqiang Cao, Feng Wang, Liandong Yu, Jing Huang, Chunyu Wu |
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| Rok vydání: | 2021 |
| Předmět: |
Carcinoma
Hepatocellular Aptamer Cell Microfluidics Biomedical Engineering Bioengineering Cell Separation Biochemistry Protein expression Circulating tumor cell Cell Line Tumor medicine Humans Cell phenotype Chemistry Liver Neoplasms General Chemistry Microfluidic Analytical Techniques medicine.disease Neoplastic Cells Circulating Surface membrane medicine.anatomical_structure Phenotype Microfluidic chip Hepatocellular carcinoma Cancer research |
| Zdroj: | Lab on a chip. 21(20) |
| ISSN: | 1473-0189 |
| Popis: | Hepatocellular carcinoma (HCC) is a harmful malady that truly debilitates human health, and hence it is of significance to isolate and on-line profile the phenotype of HCC cells for further diagnosis and therapy. We developed a novel strategy for efficient capture and in situ heterogeneous phenotype analysis of circulating tumor cells (CTCs) at the single-cell level based on surface-enhanced Raman scattering (SERS) fingerprint characteristics. Herein, a new microfluidic chip with lantern-like bypass structure was designed to capture CTCs by their large size from whole blood. Furthermore, two types of SERS-aptamer nanotags were fabricated, realizing spectral recognition of single CTCs in accordance with the surface membrane protein expression. Up to 84% of CTCs with a purity of 95% were captured from whole blood samples using the present SERS-aptamer based microfluidic chip at 20 μL min-1. The results showed that the proposed strategy can successfully identify HCC cell subtypes by SERS measurements, which was related to the clinical surface biomarkers. This may open a new avenue for serving as a powerful tool of cancer diagnosis and prognosis evaluation. |
| Databáze: | OpenAIRE |
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