Polysome activity in relation to growth and protein starvation in brains and hearts of cultured early chick embryos
| Autor: | Norman W. Klein, Audrey G. Lynch |
|---|---|
| Rok vydání: | 1978 |
| Předmět: |
Chick Embryo
Biology Biochemistry Genetics and Molecular Biology (miscellaneous) Ribosome Ribonucleases Polysome Culture Techniques Protein Deficiency medicine Animals Trypsin Ribonuclease Starvation Myocardium Brain Embryo Heart In vitro incubation Chick embryos In vitro Cell biology Biochemistry Polyribosomes Protein Biosynthesis biology.protein medicine.symptom Ribosomes |
| Zdroj: | Biochimica et biophysica acta. 519(1) |
| ISSN: | 0006-3002 |
| Popis: | In previous studies, brains but not hearts of intact early chick embryos were found to be sensitive to protein starvation. In this study, the in vitro protein synthetic activity of polysomes isolated from brains was found to be greater than those isolated from hearts. Starvation reduced the protein synthetic activity of polysomes in vitro but the extent of the reduction was approximately the same for both brains and hearts. A reduction in the amount of ribosomes as polysomes may have contributed to the lower synthetic activity of polysomes from tissues of starved embryos but not to the differences in synthetic activities between brains and hearts. In addition, neither the stability of isolated polysomes nor ribosome-associated ribonuclease activity appeared responsible for the differences observed in polysome synthetic activities. In direct relationship to the differential sensitivity of brains and hearts to starvation observed in the intact embryo, ribosomes isolated from brains of both growing and starved embryos were more readily degraded during in vitro incubation than those from hearts. |
| Databáze: | OpenAIRE |
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