Mast cell stabilizing effect of (-)-Elema-1,3,11(13)-trien-12-ol and thujopsene from Thujopsis dolabrata is mediated by down-regulation of interleukin-4 secretion in antigen-induced RBL-2H3 cells
| Autor: | Ikhoon Oh, Jongheon Shin, Kyeong Ho Kim, Chul-Hee Kim, Kung-Woo Nam, Woongchon Mar, Taehun Lee, Ki-Bong Oh |
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| Rok vydání: | 2013 |
| Předmět: |
Pharmaceutical Science
Down-Regulation Immunoglobulin E Cell Degranulation chemistry.chemical_compound Cell Line Tumor medicine Animals Thujopsene Secretion Mast Cells Interleukin 4 Pharmacology biology Chemistry Degranulation General Medicine Cupressus Mast cell biology.organism_classification Molecular biology beta-N-Acetylhexosaminidases Rats medicine.anatomical_structure Cell culture biology.protein Thujopsis Interleukin-4 Sesquiterpenes |
| Zdroj: | Biologicalpharmaceutical bulletin. 36(3) |
| ISSN: | 1347-5215 |
| Popis: | Several isolated compounds from the wood part of Thujopsis dolabrata were evaluated for their inhibitory effects against antigen-induced mast cell degranulation and interleukin-4 (IL-4) secretion, as well as IL-4 mRNA and protein expression in immunoglobulin E (IgE)-sensitized RBL-2H3 cells. Among the five isolated compounds, (-)-elema-1,3,11(13)-trien-12-ol (1) and thujopsene (2) exhibited the potent inhibitory activity against mast cell degranulation measured by β-hexosaminidase release with IC values of 27.4 µM and 25.1 µM, respectively. These compounds also inhibited the release of IL-4 (IC values of 7.0, 6.7 µM, respectively), IL-4 mRNA expression (IC values of 16.5, 7.2 µM, respectively) and IL-4 protein expression (IC values of 17.0, 9.6 µM, respectively) in antigen-induced IgE-sensitized RBL-2H3 cells. These results suggested that (-)-elema-1,3,11(13)-trien-12-ol (1) and thujopsene (2) effectively inhibits mast cell degranulation as well as IL-4 production, suggesting that these compounds from Thujopsis dolabrata can be used as candidates for IgE-mediated allergic disorders. |
| Databáze: | OpenAIRE |
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