Detection of membrane-bound tumor necrosis factor (TNF): An analysis of TNF-specific reagents
| Autor: | Carmen Kolle, Matthias Grell, Gudrun Zimmermann, Jeannette Gerspach, Heiner Böttinger, Alex Götz |
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| Rok vydání: | 2000 |
| Předmět: |
Histology
medicine.medical_treatment CHO Cells Biology Receptors Tumor Necrosis Factor law.invention Flow cytometry Mice law Cricetinae medicine Animals Humans Receptor Instrumentation medicine.diagnostic_test Tumor Necrosis Factor-alpha Cell Membrane Antibodies Monoclonal Flow Cytometry Fusion protein Molecular biology Transmembrane protein Cell biology Medical Laboratory Technology Cytokine Recombinant DNA biology.protein Tumor necrosis factor alpha Anatomy Antibody HeLa Cells |
| Zdroj: | Microscopy Research and Technique. 50:243-250 |
| ISSN: | 1097-0029 1059-910X |
| Popis: | Tumor necrosis factor (TNF) exists in two bioactive forms, the membrane integrated form and the proteolytically derived soluble cytokine. Both forms of TNF are involved in a variety of different physiological and pathophysiological situations. Here we analyzed different human and mouse TNF-specific reagents for their ability to determine the expression of membrane-expressed TNF. The data prove some antibodies to be very useful for the analysis of transmembrane TNF expression because these antibodies distinguish between the transmembrane form of TNF and soluble TNF bound to cellular TNF receptors. In addition, we found that recombinant human TNF receptor fusion proteins are advantageous tools to analyze both human and mouse transmembrane TNF expression. |
| Databáze: | OpenAIRE |
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