Genome organization of the linear plasmid, pSKL, isolated from Saccharomyces kluyveri
| Autor: | Keiko Hirai, Fumio Hishinuma |
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| Rok vydání: | 1991 |
| Předmět: |
Transcription
Genetic Sequence analysis viruses Genes Fungal Molecular Sequence Data DNA-Directed DNA Polymerase Open Reading Frames Saccharomyces Plasmid Sequence Homology Nucleic Acid Genetics Amino Acid Sequence ORFS DNA Fungal Molecular Biology Gene Genomic organization Kluyveromyces lactis Base Composition Base Sequence biology Nucleic acid sequence DNA-Directed RNA Polymerases biology.organism_classification Open reading frame Plasmids |
| Zdroj: | Molecular and General Genetics MGG. :97-106 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00273592 |
| Popis: | We have determined the complete nucleotide sequence of the linear DNA plasmid, pSKL, isolated from Saccharomyces kluyveri. Sequence analysis showed that pSKL has a high (A + T) content of 71.7%, and that there are 10 open reading frames (ORFs) larger than 250 nucleotides. All 10 ORFs were shown to be transcribed in S. kluyveri cells by S1 nuclease mapping analysis. The localization of ORFs, direction of transcription, and the predicted amino acid sequences of each ORF were quite similar to that of pGKL2, one of the killer plasmids found in Kluyveromyces lactis. The amino acid sequences of the largest two ORFs (ORF2 and ORF6) have homology with several DNA polymerases and RNA polymerases, respectively. |
| Databáze: | OpenAIRE |
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