Synergistic use of electroosmotic flow and magnetic forces for nucleic acid extraction
| Autor: | Lindsay Schneider, Rachel N. Deraney, Anubhav Tripathi |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Microfluidics
02 engineering and technology 01 natural sciences Biochemistry Buffer (optical fiber) Analytical Chemistry Magnetics Lab-On-A-Chip Devices Electrochemistry Humans Environmental Chemistry Sample preparation Spectroscopy Chromatography Human papillomavirus 18 Elution Chemistry Magnetic Phenomena Papillomavirus Infections 010401 analytical chemistry DNA Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology DNA extraction 0104 chemical sciences Reagent Magnetic bead Magnets Nucleic acid Electroosmosis 0210 nano-technology |
| Zdroj: | The Analyst. 145:2412-2419 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c9an02191d |
| Popis: | Nucleic acid sample preparation is essential for biological sample-based diagnostics. It is crucial that diagnostic tests be both specific and sensitive as to provide the most accurate diagnosis possible. Inefficient sample preparation can hinder the specificity and sensitivity of these tests since carryover contaminants can inhibit downstream processes, such as amplification. Microfluidic devices have been used previously to extract nucleic acids from a biological sample due to lower reagent volumes and ease of use. A novel microfluidic chip has been designed for nucleic acid sample preparation which combines electroosmotic flow and magnetic bead-based extraction to isolate DNA from a plasma sample. A steady electric field was incorporated into the microfluidic chip design, which when combined with a glass clover slip and a voltage differential, creates electroosmotic flow. With the goal of isolating nucleic acids into a clean, inhibitor free solution, the electroosmotic flow is the driving force and separation mechanism purifying the DNA sample captured on magnetic beads in the microfluidic chip system. Carryover volume, or the volume of unwanted sample contaminants that accompany the nucleic acids into the final elution buffer, was minimized to 0.22 ± 0.03%. In combination with magnetic bead based nucleic acid extraction techniques, a 15% increase in DNA extraction yield is reported for the microfluidic chip with the voltage applied versus without. Although the literature on nucleic acid separation in microfluidic chips is abundant, this is the first to combine microfluidic chip design, magnetic bead-based isolation and electroosmotic flow. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|