Strategies for Engineering and Rewiring Kinase Regulation
| Autor: | Kimberly A. Reynolds, Orna Resnekov, David Pincus, James W. McCormick |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Kinase Sequence analysis Allosteric regulation Eukaryota Protein engineering Computational biology Optogenetics Biology Protein Engineering Biochemistry 03 medical and health sciences 0302 clinical medicine Allosteric Regulation Sequence Analysis Protein Neoplasms Humans Kinase activity Protein Kinases Molecular Biology 030217 neurology & neurosurgery Intracellular Cell signaling pathways Signal Transduction 030304 developmental biology |
| Zdroj: | Trends in Biochemical Sciences. 45:259-271 |
| ISSN: | 0968-0004 |
| DOI: | 10.1016/j.tibs.2019.11.005 |
| Popis: | Eukaryotic protein kinases (EPKs) catalyze the transfer of a phosphate group onto another protein in response to appropriate regulatory cues. In doing so, they provide a primary means for cellular information transfer. Consequently, EPKs play crucial roles in cell differentiation and cell-cycle progression, and kinase dysregulation is associated with numerous disease phenotypes including cancer. Nonnative cues for synthetically regulating kinases are thus much sought after, both for dissecting cell signaling pathways and for pharmaceutical development. In recent years advances in protein engineering and sequence analysis have led to new approaches for manipulating kinase activity, localization, and in some instances specificity. These tools have revealed fundamental principles of intracellular signaling and suggest paths forward for the design of therapeutic allosteric kinase regulators. |
| Databáze: | OpenAIRE |
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