Further characterization of thyrotropin-displacing-activity (TDA): evidence of restricted heterogeneity
| Autor: | Witte A, Akyol B, Pickardt Cr, Hörterer L |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
Immunodiffusion medicine.drug_class Endocrinology Diabetes and Metabolism Graves' disease Clinical Biochemistry Thyroid Gland Fractionation Immunoelectrophoresis Monoclonal antibody Biochemistry Immunoglobulin G Sepharose Radioligand Assay Endocrinology Nephelometry and Turbidimetry Internal medicine medicine Humans Autoantibodies medicine.diagnostic_test biology Biochemistry (medical) General Medicine medicine.disease Chromatography Ion Exchange Graves Disease biology.protein Thyroid Stimulating Immunoglobulin Protein A Immunoglobulins Thyroid-Stimulating |
| Zdroj: | Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme. 18(12) |
| ISSN: | 0018-5043 |
| Popis: | For further characterization immunoglobulins G (IgG) of 14 patients with Graves' disease were fractionated on Protein A bound Sepharose. Sufficiently enriched IgG-subclasses were obtained despite incomplete separation. TSH displacing activity (TDA) was not homogeneously distributed in the subclasses, however IgG1 was most predominant in TDA followed by IgG3, IgG2 being essentially inactive. In two subjects TDA in whole IgG gave positive results in subclasses after fractionation, thus providing evidence, that it would be theoretically possible to move sensitivity of the method close to 100% after subfractionation. TDA was not enriched together with thyroid cell antibodies. We conclude from these results that TDA-IgG is characterized by restricted heterogeneity notwithstanding the incomplete separation of the IgG-subclasses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|