Antibiotic resistance, virulence-associated genes analysis and molecular typing of Klebsiella pneumoniae strains recovered from clinical samples

Autor: Reza Ranjbar, Amir Mirzaie
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: AMB Express
AMB Express, Vol 11, Iss 1, Pp 1-11 (2021)
ISSN: 2191-0855
Popis: Klebsiella pneumoniaeis a multidrug-resistant (MDR) opportunistic pathogen that causes nosocomial infections. Virulence analysis and molecular typing as powerful approaches can provide relevant information onK. pneumoniaeinfection. In the current study, antibiotic resistance, virulence-associated genes analysis, as well as molecular typing ofK. pneumoniaestrains were investigated. Out of 505 clinical samples collected from hospitalized patients, 100K. pneumoniaestrains were isolated by standard microbiological methods and subjected to the phenotypic and genotyping analysis. The highest prevalence of resistance was observed against ciprofloxacin (75%), trimethoprim–sulfamethoxazole (73%) and nitrofurantoin (68%). Virulence associated genes includingentB,traT,ybts, magA, iucC, htrAandrmpAwere found in 80%, 62%, 75%, 5%, 30%, 72% and 48%, of the isolates, respectively. The prevalence of biofilm-associated genes includingmrkA, fimH, andmrkDwere equally 88% for all tested isolates. Moreover, the efflux pump genes includingAcrAB, TolCandmdtKwere observed in 41 (41%), 33 (33%) and 26 (26%) of the strains respectively. A significant statistical association was observed between MDR strains and high expression of efflux pump and biofilm genes. TheK. pneumoniaestrains were differentiated into 11 different genetic patterns using the repetitive element sequence-based PCR (rep-PCR) technique. High prevalence of resistance, presence of various virulence factors, high level of efflux pump, and biofilm gene expression in diverse clones ofK. pneumoniaestrains pose an important health issue in clinical settings.
Databáze: OpenAIRE
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