| Autor: |
Boris Vojnovic, Tony Ng, Simon Ameer-Beg, Daniel R. Matthews, Klaus Suhling, R G Newman, Melanie Keppler, Malcolm Irving, Paul R. Barber, Leo M. Carlin, G P Pierce |
| Jazyk: |
angličtina |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
Progress in Biomedical Optics and Imaging - Proceedings of SPIE. 6859 |
| ISSN: |
1605-7422 |
| Popis: |
An automated high-content screening microscope has been developed which uses fluorescence anisotropy imaging and fluorescence lifetime microscopy to identify Förster resonant energy transfer between eGFP and mRPF1 in drug screening assays. A wide-field polarization resolved imager is used to simultaneously capture the parallel and perpendicular components of both eGFP and mRFP1 fluorescence emission to provide a high-speed measurement of acceptor depolarization. Donor excited state lifetime measurements performed using laser scanning microscopy is then used to determine the FRET efficiency in a particular assay. A proof-of-principle assay is performed using mutant Jurkat human T-cells to illustrate the process by which FRET is first identified and then quantified by our high-content screening system. © 2008 Copyright SPIE - The International Society for Optical Engineering. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
