Intestinal handling-induced mast cell activation and inflammation in human postoperative ileus
| Autor: | F O The, R J Bennink, W M Ankum, M R Buist, O R C Busch, D J Gouma, S van der Heide, R M van den Wijngaard, W J de Jonge, G E Boeckxstaens |
|---|---|
| Přispěvatelé: | Amsterdam institute for Infection and Immunity, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Nuclear Medicine, Other Research, Obstetrics and Gynaecology, Surgery, Gastroenterology and Hepatology |
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Transcription Genetic SURGERY Lymphocyte DEGRANULATION Inflammation Tryptase Cell Degranulation Ileus MANIPULATION Abdomen Medicine Humans Mast Cells MUSCULARIS Interleukin 6 Intraoperative Complications Aged biology business.industry COLON-CANCER Gastroenterology Degranulation Middle Aged QUANTIFICATION Mast cell RANDOMIZED-TRIAL CROHNS-DISEASE Gastroenteritis Nitric oxide synthase MICE medicine.anatomical_structure biology.protein RISK-FACTORS Female Stress Mechanical medicine.symptom business Intracellular |
| Zdroj: | Gut, 57(1), 33-40. BMJ Publishing Group Gut, 57(1), 33-40. BMJ PUBLISHING GROUP |
| ISSN: | 0017-5749 |
| Popis: | Background: Murine postoperative ileus results from intestinal inflammation triggered by manipulation-induced mast cell activation. As its extent depends on the degree of handling and subsequent inflammation, it is hypothesised that the faster recovery after minimal invasive surgery results from decreased mast cell activation and impaired intestinal inflammation.Objective: To quantify mast cell activation and inflammation in patients undergoing conventional and minimal invasive surgery.Methods: (1) Mast cell activation (ie, tryptase release) and pro-inflammatory mediator release were determined in peritoneal lavage fluid obtained at consecutive time points during open, laparoscopic and transvaginal gynaecological surgery. (2) Lymphocyte function-associated antigen-1 (LFA-1), intercellular adhesion molecule-1 (ICAM-1) and inducible nitric oxide synthase (iNOS) mRNA as well as leucocyte influx were quantified in non-handled and handled jejunal muscle specimens collected during biliary reconstructive surgery. (3) Intestinal leucocyte influx was assessed by 99mTc-labelled leucocyte single photon emission computed tomography (SPECT)-computed tomography (CT) scanning before and after abdominal or vaginal hysterectomy.Results: (1) Intestinal handling during abdominal hysterectomy resulted in an immediate release of tryptase followed by enhanced interleukin 6 (IL6) and IL8 levels. None of the mediators increased during minimal invasive surgery except for a slight increase in IL8 during laparoscopic surgery. (2) Jejunal ICAM-1 and iNOS mRNA transcription as well as leucocyte recruitment were increased after intestinal handling. (3) Leucocyte scanning 24 h after surgery revealed increased intestinal activity after abdominal but not after vaginal hysterectomy.Conclusions: This study demonstrates that intestinal handling triggers mast cell activation and inflammation associated with prolonged postoperative ileus. These results may partly explain the faster recovery after minimal invasive surgery and encourage future clinical trials targeting mast cells to shorten postoperative ileus. |
| Databáze: | OpenAIRE |
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