Characterization and expression of theHex 110 gene encoding a glutamine-rich hexamerin in the honey bee,Apis mellifera
| Autor: | Márcia Maria Gentile Bitondi, Adriana D. Cunha, Karina R. Guidugli, Adriana Mendes do Nascimento, Zilá Luz Paulino Simões, Francis M. F. Nunes |
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| Rok vydání: | 2006 |
| Předmět: |
animal structures
food.ingredient Physiology Glutamine Protein subunit medicine.medical_treatment Molecular Sequence Data Biology Bioinformatics Biochemistry food Hemolymph Royal jelly medicine Animals Storage protein Amino Acid Sequence RNA Messenger Peptide sequence chemistry.chemical_classification Fatty Acids Ovary fungi Pupa Gene Expression Regulation Developmental Hemocyanin General Medicine Honey bee Bees Worker bee chemistry Larva Insect Science Insect Proteins Pollen Female |
| Zdroj: | Archives of Insect Biochemistry and Physiology. 63:57-72 |
| ISSN: | 1520-6327 0739-4462 |
| DOI: | 10.1002/arch.20142 |
| Popis: | An N-terminal amino acid sequence of a previously reported honey bee hexamerin, HEX 110 [Danty et al., Insect Biochem Mol Biol 28:387-397 (1998)], was used as reference to identify the predicted genomic sequence in a public GenBank database. In silico analysis revealed an ORF of 3,033 nucleotides that encompasses eight exons. The conceptual translation product is a glutamine-rich polypeptide with a predicted molecular mass of 112.2 kDa and pI of 6.43, which contains the conserved M and C hemocyanin domains. Semiquantitative and quantitative RT-PCR with specific primers allowed for an analysis of mRNA levels during worker bee development and under different physiological conditions. Concomitantly, the abundance of the respective polypeptide in the hemolymph was examined by SDS-PAGE. Hex 110 transcripts were found in high levels during the larval stages, then decreased gradually during the pupal stage, and increased again in adults. HEX 110 subunits were highly abundant in larval hemolymph, decreased at the spinning-stage, and remained at low levels in pupae and adults. In 5th instar larvae, neither starvation nor supplementation of larval food with royal jelly changed the Hex 110 transcript levels or the amounts of HEX 110 subunit in hemolymph. In adult workers, high levels of Hex 110 mRNA, but not of the respective subunit, were related to ovary activation, and also to the consumption of a pollen-rich diet. |
| Databáze: | OpenAIRE |
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