| Autor: |
Alessandro Del Cuore, Rosaria Maria Pipitone, Alessandra Casuccio, Marco Mazzola, Maria Grazia Puleo, Gaetano Pacinella, Renata Riolo, Carlo Maida, Tiziana Chiara, Domenico Raimondo, Rossella Zito, Giulia Lupo, Luisa Agnello, Marcello Ciaccio, Stefania Grimaudo, Antonino Tuttolomondo |
| Přispěvatelé: |
Alessandro Del Cuore, Rosaria Maria Pipitone, Marco Mazzola, Maria Grazia Puleo, Gaetano Pacinella, Renata Riolo, Carlo Maida, Alessandra Casuccio, Tiziana Di Chiara, Domenico Di Raimondo, Rossella Zito, Giulia Lupo, Luisa Agnello, Marcello Ciaccio, Stefania Grimaudo, Antonino Tuttolomondo |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Popis: |
Background: Diabetic foot is a significant cause of morbidity in diabetic patients, with a rate that is approximatelytwice that of patients without foot ulcers. “Metabolic memory” represents the epigenetic changes induced by chronic hyperglycaemia, despite the correction of the glucose levels themselves. These epigenetic modifications appear to perpetuate the damage caused by persistently elevated glucose levels even in their absence, acting at various levels, mostly affecting the molecular processes of diabetic ulcer healing. Methods: The aim of our cross-sectional study was to analyse a cohort of patients with diabetes with and without lower limb ulcers. We examined the effects of epigenetic changes on miRNA 126, 305, and 217 expression and the frequency of the SNPs of genes encoding inflammatory molecules (e.g., IL-6 and TNF-alpha) and their correlations with serum levels of proangiogenic molecules (e.g., ENOS, VEGF and HIF-1alpha) and several adipokines as well as with endothelial dysfunction, assessed noninvasively by reactive hyperaemia peripheral artery tonometry . Between March 2021 and June 2022, 110 patients were enrolled into the study: 50 diabetic patients with diabetic foot injuries, 40 diabetic patients without ulcerative complications and 20 nondiabetic patients as the control group. Results: Diabetic subjects with lower limb ulcerative lesions exhibited higher levels of inflammatory cytokines, such as VEGF (191.40±200 pg/mL vs. 98.27±56.92 pg/mL vs. 71.01±52.96 pg/mL; p=0.22), HIF-1alpha (40.18±10.80 ng/mL vs. 33.50±6.16 ng/mL vs. 33.85±6.84 ng/mL; p=0.10), and Gremlin-1 (1.72±0.512 ng/mL vs. 1.31±0.21 ng/mL vs. 1.11±0.19 ng/mL; p< 0.0005), than those without lower limb ulcers and healthy controls. Furthermore, we observed that miR-217-5p and miR-503-5p were 2.19-fold (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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