Millisecond duration pulses for flow-through electro-induced protein extraction from E. coli and associated eradication
| Autor: | Mathilde Coustets, Bojidar Galutzov, V. Ganeva, Justin Teissié |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Biophysics
Analytical chemistry Buffers Chemical Fractionation Biology Cell wall Electric field Protein purification Escherichia coli Electrochemistry Physical and Theoretical Chemistry Leakage (electronics) Millisecond Escherichia coli Proteins Electroporation Cell Membrane Temperature Pulse duration Electrochemical Techniques General Medicine Recombinant Proteins Membrane Muramidase Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) |
| Zdroj: | Bioelectrochemistry. 103:82-91 |
| ISSN: | 1567-5394 |
| Popis: | Pulsed electric fields are used to induce membrane permeabilization on cells. In the case of species with cell wall (yeasts, microalgae), it was previously shown that when the pulse duration was several ms long, this resulted in a cytoplasmic soluble protein slow leakage. In this work, we show that a similar consequence can be obtained with different strains of E. coli. Experimental evidences of a resulting wall alteration are described. Pre-industrial flow process pilots are used. As the membrane electropermeabilization can be irreversible by applying a proper choice of the pulse parameters, this approach is used for bacterial inactivation in flow process. It is observed that sub-millisecond pulse trains are more cost effective than longer ones. |
| Databáze: | OpenAIRE |
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