HER2/neu protein expression and fine needle breast aspiration from Argentinean patients with non-palpable breast lesions
| Autor: | Hector Nardi, Maria Marta Corte, Gabriela A. Balogh, Wendy Gabriela Domínguez, Esteban Vincent, Hector Montero |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Breast biopsy
Cancer Research Pathology medicine.medical_specialty GENE EXPRESSION CIENCIAS MÉDICAS Y DE LA SALUD Ciencias de la Salud HER2/NEU HER2/neu HER2/NEW purl.org/becyt/ford/3.3 [https] Breast cancer Immunology and Microbiology (miscellaneous) medicine skin and connective tissue diseases neoplasms medicine.diagnostic_test biology business.industry Cancer Articles General Medicine medicine.disease NON-PALPABLE BREAST CARCINOMA body regions Otras Ciencias de la Salud BREAST FINE NEEDLE ASPIRATE surgical procedures operative Fine-needle aspiration Monoclonal Cancer cell biology.protein Immunohistochemistry purl.org/becyt/ford/3 [https] BREAST FINE NEEDLE ASPIRATION business |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | The objective of this pilot project was to investigate whether the breast fine needle aspiration (FNA) technique is a useful tool for determining the increased risk of breast cancer in patients with non-palpable breast lesions. FNA is a minimally invasive technique that isolates mammary epithelial cells from breast cells in the suspicious region. In this study, two FNA samples were collected from 12 patients. The level of HER2/neu expression at the mRNA level (in serum) was measured in each patient. As gene amplification is characteristic of cancer cells and may assist in diagnosis and prognostic assessment, it is crucial that gene amplification of HER2/neu in patients with non-palpable breast lesions is compared to breast biopsy results. In serum, the level of HER2/neu was determined by ELISA assay. Gene amplification was determined by PCR and confirmed by IHC employing monoclonal ERRB2 in the FNA sample. The results indicate that FNA has a good correlation with breast biopsy. FNA combined with mammographic imaging is a strong tool for determining favorable treatment options for patients. Fil: Domínguez, Wendy Gabriela. Texas A&M University; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina Fil: Nardi, Héctor. Hospital Interzonal Dr José Penna. Departamento de Ginecología. Bahía Blanca; Argentina Fil: Montero, Héctor. Hospital Español. Departamento de Ginecología; Argentina Fil: Vincent, Esteban. Hospital de la Asociación Médica de Bahía Blanca. Departamento de Ginecología. Bahía Blanca; Argentina Fil: Corte, María Marta. Laboratorio de Patología. Bahía Blanca; Argentina Fil: Balogh, Gabriela Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiarida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiarida; Argentina |
| Databáze: | OpenAIRE |
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