Gadolinium-enhanced cardiac MR exams of human subjects are associated with significant increases in the DNA repair marker 53BP1, but not the damage marker γH2AX

Autor: David F. Kallmes, Sylvain V. Costes, Tamara M. Hudson, Dana Schroeder, Jennifer S. McDonald, Philip M. Young, Jacob B. Ekins, Anthony S. Tin, Robert J. McDonald, Aiming Lu, Ramanathan Kadirvel, Scott H. Kaufmann, Kevin M. Kallmes
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Pathology
DNA Repair
Physiology
medicine.medical_treatment
lcsh:Medicine
Gadolinium
030204 cardiovascular system & hematology
Biochemistry
Diagnostic Radiology
Histones
White Blood Cells
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Lymphocytes
Prospective Studies
Prospective cohort study
lcsh:Science
Multidisciplinary
medicine.diagnostic_test
Radiology and Imaging
Heart
Middle Aged
Magnetic Resonance Imaging
Body Fluids
Nucleic acids
Chemistry
Blood
Physical Sciences
Female
Cellular Types
Anatomy
Cardiomyopathies
Tumor Suppressor p53-Binding Protein 1
Research Article
Chemical Elements
Adult
medicine.medical_specialty
DNA damage
Imaging Techniques
Immune Cells
Immunology
Cardiology
Malignancy
Research and Analysis Methods
Drug Absorption
Peripheral blood mononuclear cell
03 medical and health sciences
Diagnostic Medicine
medicine
Genetics
Humans
Clinical significance
Pharmacokinetics
Aged
Retrospective Studies
Pharmacology
Chemotherapy
Blood Cells
Biology and life sciences
business.industry
lcsh:R
Correction
Magnetic resonance imaging
DNA
Cell Biology
medicine.disease
Radiation therapy
030104 developmental biology
lcsh:Q
business
Biomarkers
DNA Damage
Zdroj: PLoS ONE, Vol 13, Iss 1, p e0190890 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Magnetic resonance imaging is considered low risk, yet recent studies have raised a concern of potential damage to DNA in peripheral blood leukocytes. This prospective Institutional Review Board-approved study examined potential double-strand DNA damage by analyzing changes in the DNA damage and repair markers γH2AX and 53BP1 in patients who underwent a 1.5 T gadolinium-enhanced cardiac magnetic resonance (MR) exam. Sixty patients were enrolled (median age 55 years, 39 males). Patients with history of malignancy or who were receiving chemotherapy, radiation therapy, or steroids were excluded. MR sequence data were recorded and blood samples obtained immediately before and after MR exposure. An automated immunofluorescence assay quantified γH2AX or 53BP1 foci number in isolated peripheral blood mononuclear cells. Changes in foci number were analyzed using the Wilcoxon signed-rank test. Clinical and MR procedural characteristics were compared between patients who had a >10% increase in γH2AX or 53BP1 foci numbers and patients who did not. The number of γH2AX foci did not significantly change following cardiac MR (median foci per cell pre-MR = 0.11, post-MR = 0.11, p = .90), but the number of 53BP1 foci significantly increased following MR (median foci per cell pre-MR = 0.46, post-MR = 0.54, p = .0140). Clinical and MR characteristics did not differ significantly between patients who had at least a 10% increase in foci per cell and those who did not. We conclude that MR exposure leads to a small (median 25%) increase in 53BP1 foci, however the clinical relevance of this increase is unknown and may be attributable to normal variation instead of MR exposure.
Databáze: OpenAIRE
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