Uncovering Distinct Primary Vaccination-Dependent Profiles in Human Bordetella Pertussis Specific CD4+ T-Cell Responses Using a Novel Whole Blood Assay

Autor: Dimitri D Diavatopoulos, Eleonora E. Lambert, Cécile A. C. M. van Els, Maxime Duijst, Véronique Corbière, Prashanna Balaji Venkatasubramanian, Martijn A. Huynen, Pauline Versteegen, Françoise Mascart, Elles Simonetti, Jacqueline A. M. van Gaans-van den Brink, Guy A. M. Berbers
Rok vydání: 2020
Předmět:
0301 basic medicine
Th cytokines
Bordetella pertussis
medicine.medical_treatment
Immunology
Primary vaccination
lnfectious Diseases and Global Health Radboud Institute for Molecular Life Sciences [Radboudumc 4]
lcsh:Medicine
Priming (immunology)
vaccination background
Article
CD4+ T-cells
03 medical and health sciences
Vaccination background
0302 clinical medicine
Whole blood assay
Immunologie
Drug Discovery
Medicine
Pharmacology (medical)
Booster vaccination
Whole blood
Pharmacology
biology
business.industry
lcsh:R
Metabolic Disorders Radboud Institute for Molecular Life Sciences [Radboudumc 6]
biology.organism_classification
Sciences biomédicales
In vitro
whole blood assay
3. Good health
Th polarization
Vaccination
booster vaccination
030104 developmental biology
Infectious Diseases
Cytokine
business
Intracellular
030215 immunology
Zdroj: Vaccines (Basel), 8
Vaccines
Volume 8
Issue 2
Vaccines, 8 (2
Vaccines, Vol 8, Iss 225, p 225 (2020)
Vaccines (Basel), 8, 2
ISSN: 2076-393X
Popis: To advance research and development of improved pertussis vaccines, new immunoassays are needed to qualify the outcome of Bordetella pertussis (Bp) specific CD4+ T-cell differentiation. Here, we applied a recently developed whole blood assay to evaluate Bp specific CD4+ T-cell responses. The assay is based on intracellular cytokine detection after overnight in vitro Bp antigen stimulation of diluted whole blood. We show for the first time that CD4+ T-cell memory of Th1, Th2, and Th17 lineages can be identified simultaneously in whole blood. Participants ranging from 7 to 70 years of age with different priming backgrounds of whole-cell pertussis (wP) and acellular pertussis (aP) vaccination were analyzed around an acellular booster vaccination. The assay allowed detection of low frequent antigen-specific CD4+ T-cells and revealed significantly elevated numbers of activated and cytokine-producing CD4+ T-cells, with a significant tendency to segregate recall responses based on primary vaccination background. A stronger Th2 response hallmarked an aP primed cohort compared to a wP primed cohort. In conclusion, analysis of Bp specific CD4+ T-cell responses in whole blood showed separation based on vaccination background and provides a promising tool to assess the quantity and quality of CD4+ T-cell responses induced by vaccine candidates.
SCOPUS: ar.j
info:eu-repo/semantics/published
Databáze: OpenAIRE
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