Expression imbalance of transcription factors GATA-3 and T-bet in post-resuscitation myocardial immune dysfunction in a porcine model of cardiac arrest
| Autor: | Da Zhang, Jian Zhang, Wei Gu, Zhijun Guo, Wen Peng Yin, Chun Sheng Li, Xiao Min Hou |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Swine medicine.medical_treatment Blotting Western Enzyme-Linked Immunosorbent Assay GATA3 Transcription Factor CD8-Positive T-Lymphocytes Emergency Nursing Return of spontaneous circulation Real-Time Polymerase Chain Reaction Flow cytometry Andrology Interferon-gamma Th2 Cells Interferon Animals Medicine RNA Messenger Cardiopulmonary resuscitation Transcription factor medicine.diagnostic_test business.industry Myocardium Interleukin Th1 Cells Flow Cytometry Cardiopulmonary Resuscitation Heart Arrest Blot Gene Expression Regulation Immunology Emergency Medicine Swine Miniature Interleukin-4 T-Box Domain Proteins Cardiology and Cardiovascular Medicine business CD8 medicine.drug |
| Zdroj: | Resuscitation. 84:848-853 |
| ISSN: | 0300-9572 |
| Popis: | This study investigated whether an imbalance in Th1/Th2 cells is involved in the post-resuscitation myocardial immune dysfunction.26 Wuzhishan miniature pigs were randomly divided into return of spontaneous circulation (ROSC) group (n=20) and sham-operated group (n=6), 20 pigs were subjected to 8min of electrically induced cardiac arrest, After successful ROSC, the 16 surviving pigs were randomly assigned to be sacrificed (n=8 per group) at 12 and 24h after ROSC, respectively. CD4(+) and CD8(+) lymphocyte subsets were determined by flow cytometry, interleukin (IL)-4 and interferon (IFN)-γ in the myocardium were measured by ELISA, and protein and mRNA levels of GATA-3 and T-bet were detected in the myocardium by Western blotting and quantitative real-time PCR in the post-ROSC group (n=8 per group) at 12 and 24h after ROSC and sham-operated group (n=6) at 24h after ROSC, respectively.CD4(+) lymphocyte subsets were significantly lower in the post-ROSC group compared with the sham-operated group (P0.05) at 12 and 24h after ROSC. The levels of myocardium IFN-γ were markedly increased, while IL-4 was significantly decreased in the post-ROSC group compared with the sham-operated group (P0.05) at 12 and 24h after ROSC. Protein expression and mRNA levels of T-bet were markedly increased in the myocardium of pigs in the post-ROSC group compared with the sham-operated group (P0.05) at 12 and 24h after ROSC, while GATA-3 was significantly reduced (P0.05).The myocardial immune dysfunction induced by the change in expression levels of the transcription factors GATA-3 and T-bet may be involved in the process of post-resuscitation myocardial injury in a porcine model of cardiac arrest. |
| Databáze: | OpenAIRE |
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