36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells
| Autor: | Barbara Torsello, Giorgio Bovo, Silvia Bombelli, Sofia De Marco, C Grasselli, Roberto A. Perego, Giuseppe Lucarelli, Cristina Bianchi, Ingrid Cifola, Guido Strada |
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| Přispěvatelé: | Bombelli, S, Torsello, B, De Marco, S, Lucarelli, G, Cifola, I, Grasselli, C, Strada, G, Bovo, G, Perego, R, Bianchi, C |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
lipid storage 0301 basic medicine Gene isoform renal cell carcinoma annexin A3 lipid storage Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor Adipocyte medicine Humans Gene silencing Carcinoma Renal Cell Annexin A3 Aged ccRCC isoforms Lipid metabolism Lipid Metabolism medicine.disease Kidney Neoplasms Neoplasm Proteins Cell biology Isoenzymes Clear cell renal cell carcinoma 030104 developmental biology chemistry Cell culture Adipogenesis 030220 oncology & carcinogenesis Female |
| Zdroj: | The American journal of pathology 190 (2020): 2317–2326. doi:10.1016/j.ajpath.2020.08.008 info:cnr-pdr/source/autori:Bombelli, Silvia; Torsello, Barbara; De Marco, Sofia; Lucarelli, Giuseppe; Cifola, Ingrid; Grasselli, Chiara; Strada, Guido; Bovo, Giorgio; Perego, Roberto A.; Bianchi, Cristina/titolo:36-kDa Annexin A3 Isoform Negatively Modulates Lipid Storage in Clear Cell Renal Cell Carcinoma Cells/doi:10.1016%2Fj.ajpath.2020.08.008/rivista:The American journal of pathology (Print)/anno:2020/pagina_da:2317/pagina_a:2326/intervallo_pagine:2317–2326/volume:190 |
| DOI: | 10.1016/j.ajpath.2020.08.008 |
| Popis: | The adipocyte-like morphology of clear cell renal cell carcinoma (ccRCC) cells results from a grade-dependent neutral lipid accumulation; however, the molecular mechanism and role in renal cancer progression have yet to be clarified. ccRCC shows a gene expression signature consistent with adipogenesis, and the phospholipid-binding protein annexin A3 (AnxA3), a negative regulator of adipocyte differentiation, is down-regulated in RCC and shows a differential expression pattern for two isoforms of 36 and 33 kDa. Using primary cell cultures and cell lines, we investigated the involvement of AnxA3 isoforms in lipid storage modulation of ccRCC cells. We found that the increased accumulation of lipids into ccRCC cells correlated with a decrease of the 36/33 isoform ratio. Treatment with adipogenic medium induced a significant increment of lipid storage in ccRCC cells that had a low 36-kDa AnxA3 expression and 36/33 ratio. The 36-kDa AnxA3 silencing in ccRCC cells increased lipid storage induced by adipogenic medium. These data suggest that 36-kDa AnxA3 negatively modulates the response to adipogenic treatment and may act as negative regulator of lipid storage in ccRCC cells. The subcellular distribution of AnxA3 in the cellular endocytic compartment suggests its involvement in modulation of vesicular trafficking, and it might serve as a putative mechanism of lipid storage regulation in ccRCC cells, opening novel translational outcomes. |
| Databáze: | OpenAIRE |
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