Molecular analysis of the bcr rearrangement in a case of Ph‘-negative blastic crisis of Ph’-positive chronic myelogenous leukemia
| Autor: | Maurizio Miglino, Letizia Canepa, Anna Maria Ferraris, Gian Franco Gaetani, Cecilia Melani, Mario Sessarego |
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| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty Chromosomes Human Pair 22 Trisomy Blastic Phase Biology Translocation Genetic chemistry.chemical_compound Bone Marrow Leukemia Myelogenous Chronic BCR-ABL Positive hemic and lymphatic diseases medicine Humans Philadelphia Chromosome Gene Rearrangement Chromosomes Human Pair 13 breakpoint cluster region Cytogenetics Chromosome DNA Neoplasm Hematology General Medicine Gene rearrangement medicine.disease Molecular biology Molecular analysis Blotting Southern chemistry Karyotyping Cancer research Blast Crisis Chromosomes Human Pair 9 DNA Chronic myelogenous leukemia |
| Zdroj: | European Journal of Haematology. 42:32-37 |
| ISSN: | 1600-0609 0902-4441 |
| DOI: | 10.1111/j.1600-0609.1989.tb00243.x |
| Popis: | We describe here a patient with Ph'-positive chronic myelogenous leukemia (CML) who developed a Ph'-negative blastic crisis. The blast DNA was analyzed on two different occasions, at the beginning of the blastic phase and at the end, shortly before the patient's death. Although cells from both samples had no Ph' chromosome marker (not even a masked one) we could detect a rearrangement of the bcr gene in the second DNA sample, using a '3'-bcr' probe. The same probe and a '5'-bcr' probe failed to detect any rearranged band in the first DNA sample. No rearrangement was identified at the c-myc and N-ras loci, while a slight c-myc amplification was evident in both DNA samples tested. |
| Databáze: | OpenAIRE |
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