Ischemic injury activates PTHrP and PTH1R expression in human ventricular cardiomyocytes

Autor: Egidio Stigliano, Sandra Pasquini, Roberta Fiaccavento, Giovanni Monego, Luciano Potena, Paolo Di Nardo, Vincenzo Arena, Franco O. Ranelletti, Giorgio Arpesella, Arnaldo Capelli, Ornella Leone
Přispěvatelé: G. Monego, V. Arena, S. Pasquini, E. Stigliano, R. Fiaccavento, O. Leone, G. Arpesella, L. Potena, F.O. Ranelletti, P. Di Nardo, A. Capelli
Rok vydání: 2008
Předmět:
Male
Physiology
Myocardial Ischemia
Parathyroid hormone
Fluorescent Antibody Technique
Settore MED/11 - Malattie dell'Apparato Cardiovascolare
Muscle hypertrophy
Medicine
Myocyte
Myocytes
Cardiac
Receptor
Child
cellular distribution
Aged
80 and over
Middle Aged
Immunohistochemistry
coronary artery atherosclerosis
Child
Preschool
Female
Hypertrophy
Left Ventricular
hypertrophy
Cardiology and Cardiovascular Medicine
ischemic injury
hormones
hormone substitutes
and hormone antagonists
Adult
medicine.medical_specialty
heart muscle ischemia
Adolescent
cardiac
PTHrP
Heart Ventricles
Ischemia
heart muscle cell
heart
Contractility
Young Adult
Physiology (medical)
Internal medicine
Humans
right ventricular
Aged
Receptor
Parathyroid Hormone
Type 1
PTH1R
Hypertrophy
Right Ventricular
business.industry
Parathyroid Hormone-Related Protein
medicine.disease
Endocrinology
parathyroid hormone receptor 1
adolescent
adult
hypertrophy
left ventricular
hypertrophy
right ventricular
left ventricular
business
Immunostaining
Zdroj: Basic research in cardiology. 104(4)
ISSN: 1435-1803
Popis: The PTHrP/PTH1R signalling system induces calciotropic and myorelaxant effects on the vascular system and plays critical roles in the development of the cardiovascular system. In animal models, PTHrP exerts important effects on heart rate and contractility, particularly under ischemic conditions, while, in human hearts, the expression of PTHrP by cardiomyocytes remains to be defined in both normal and ischemic conditions. The present study has been conducted on 101 autoptical cases and confirmed on nine explanted hearts in order to analyze the expression of the PTHrP/PTH1R system by ventricular myocardium in respect to morphological aspects of the myocardial ischemic damage, myofiber hypertrophy and disarray, coronarosclerosis, age and sex. Immunohistochemistry showed positive cytoplasmic immunostaining for both PTHrP and PTH1R in ventricular cardiomyocytes. The expression levels of the PTHrP/PTH1R system resulted significantly increased (P = 0.0008 and P < 0.0001, respectively) in association with the myocardial ischemic damage and the presence of cardiomyocyte hypertrophy (P = 0.02 and P = 0.009 respectively). Conversely, increased expression levels of PTHrP alone were observed in myofiber disarray (P = 0.04), whereas PTH1R was augmented in coronarosclerosis (P = 0.004) and age (P = 0.001). Taken together, these results demonstrate that human ventricular cardiomyocytes express PTHrP and PTH1R and suggest that the activation of the PTHrP/PTH1R system could represent an aspect of the embryonic gene program typically reactivated by the myocardium when subjected to ischemia and/or hypertrophy.
Databáze: OpenAIRE
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