Cell morphology impact on the set-up of electroporation protocols for in-suspension and adhered breast cancer cells

Autor:	C. E. Vera-Tizatl, P Talamás-Rohana, A L Vera-Tizatl, B Chávez-Munguía, S. A. Rodriguez-Cuevas, Arturo Vera-Hernández, Lorenzo Leija-Salas
Rok vydání:	2020
Předmět:	Electrochemotherapy Cell Survival Cell Biophysics Medicine (miscellaneous) Breast Neoplasms Cell morphology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Suspensions Cell Adhesion medicine Humans Propidium iodide Viability assay Chemistry Electroporation General Medicine Irreversible electroporation medicine.anatomical_structure Cell culture 030220 oncology & carcinogenesis MCF-7 Cells Cancer research 030217 neurology & neurosurgery
Zdroj:	Electromagnetic Biology and Medicine. 39:323-339
ISSN:	1536-8386 1536-8378
Popis:	In order to establish cancer-type-specific electroporation protocols for breast cancer, electroporation was performed in vitro in two modalities: in-suspension and adhered cells. Electroporation of cell suspensions was carried out through commercial electroporation cuvettes whereas a novel electrode for electroporation of adhered cells was designed and manufactured aimed to preserve cell structure, to provide a closer model to an in vivo scenario, and as a means to visualize the mechanical effects of electroporation on the cell membrane by using scanning electron microscopy. Electroporation protocols and electric field thresholds were predicted in silico and experimentally tuned through propidium iodide uptake and cell viability. Three breast-cancer cell lines (BT-20, MCF-7 and HCC1419) and a non-cancerous cell line (BEAS-2B) were used. Cancerous cells responded differently to electroporation depending on the electric parameters, cell histology, the cell culture modality, and the cell morphology (membrane thickness mainly), which was evaluated trough confocal and transmission electron microscopy. Particularly, it was found that electrochemotherapy may represent a promising alternative as an adjuvant treatment of metastatic breast tumours, and as a neoadjuvant therapy for Her2/neu tumours. Oppositely, triple negative breast tumours may show a high sensitivity to electroporation and therefore, they could be efficiently treated with irreversible electroporation. On the other hand, noncancerous cells demanded the highest voltage in both cell culture modalities in order to be electroporated. Hence, these cells in suspension may provide a reliable, easy-to-perform, low-cost model for the development of electroporation protocols for eradication of healthy tissue around a tumour in a safety margin.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9a2e0c3512a0b949a36f82c4657d8ce https://doi.org/10.1080/15368378.2020.1799387 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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