Serum of patients with antiphospholipid syndrome induces adhesion molecules in endothelial cells

Autor: Martin Aringer, Bettina Engel, H. E. Schröder, B. Roch, Henning Morawietz, Stefan R. Bornstein, Gregor Muller
Přispěvatelé: University of Zurich, Morawietz, Henning
Rok vydání: 2017
Předmět:
0301 basic medicine
Adult
Male
10265 Clinic for Endocrinology and Diabetology
Vascular Cell Adhesion Molecule-1
610 Medicine & health
030204 cardiovascular system & hematology
Biology
2705 Cardiology and Cardiovascular Medicine
Umbilical vein
Andrology
03 medical and health sciences
0302 clinical medicine
Western blot
Annexin
Antiphospholipid syndrome
Gene expression
Internal Medicine
medicine
Human Umbilical Vein Endothelial Cells
Humans
RNA
Messenger
Blood Coagulation
Cells
Cultured
medicine.diagnostic_test
Cell adhesion molecule
General Medicine
Middle Aged
medicine.disease
Antiphospholipid Syndrome
Intercellular Adhesion Molecule-1
Pathophysiology
Up-Regulation
Endothelial stem cell
030104 developmental biology
2724 Internal Medicine
Case-Control Studies
Immunology
Female
Cardiology and Cardiovascular Medicine
Zdroj: Atherosclerosis. Supplements. 30
ISSN: 1878-5050
Popis: Objective The antiphospholipid syndrome (APS) is a systemic auto-immune disease with an unclear pathophysiology. The aim of our study was to understand the development of APS on a cellular level. Therefore, we analyzed the influence of human serum of APS patients on endothelial expression of specific genes and proteins in comparison to a control group. Methods In this study, we analyzed the expression of ICAM-1, VCAM-1, E-selectin and annexin V in primary cultures of human umbilical vein endothelial cells (HUVEC) in response to 10% (v/v) serum of control patients (n = 6), patients with systemic lupus erythematosus (SLE) and no APS (n = 4) or APS patients (n = 9) for 24 h. Total RNA was prepared from confluent endothelial cell layers and mRNA expression of ICAM-1, VCAM-1 and E-selectin was analyzed by reverse transcription polymerase-chain reaction (RT-PCR). The protein expression was determined by Western blot. Serum protein concentrations of soluble forms of adhesion molecules sICAM-1 and sVCAM-1 were quantified by ELISA. Gene expression data were correlated with clinical parameters. Results The mRNA expression of ICAM-1 was increased in cells incubated with serum from APS patients (166 ± 22% of control; P = 0.023). Serum of patients with (SLE)/no APS caused a 1.4-fold higher ICAM-1 mRNA level. Western blot analysis showed an increase in protein expression of adhesion molecules ICAM-1 (260 ± 49%; P = 0.011) and VCAM-1 (357 ± 97%; P = 0.023) in cells that were incubated with serum from APS patients. Plasma analysis showed elevated levels of sVCAM-1 in APS patients (189 ± 34%; P = 0.045) compared to the levels measured in the control group. The sVCAM-1 plasma level was correlating with the frequency of abortions. Conclusion An augmented expression of endothelial adhesion molecules is involved in the pathophysiology of patients with antiphospholipid syndrome.
Databáze: OpenAIRE
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