Proliferation responses to HIVp24 during antiretroviral therapy do not reflect improved immune phenotype or function
| Autor: | Robert Asaad, Sandra J. Lee, Zhan Xu, Judy Lieberman, Hernan Valdez, Michael M. Lederman, Brooke Harnisch, Bruce K. Patterson, Kathy Medvik, Christoph Lange, Alan L. Landay |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Immunology HIV Core Protein p24 HIV Infections chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Lymphocyte Activation Virus Replication Immunophenotyping Immune system T-Lymphocyte Subsets Antiretroviral Therapy Highly Active Immunopathology Humans Immunology and Allergy biology Vaccination CD28 Middle Aged Infectious Diseases Granzyme Perforin Chronic Disease HIV-1 Granzyme A biology.protein RNA Viral Cell Division CD8 |
| Zdroj: | AIDS. 18:605-613 |
| ISSN: | 0269-9370 |
| DOI: | 10.1097/00002030-200403050-00004 |
| Popis: | To ascertain whether lymphoproliferation (LP) responses to HIVp24 in chronically infected patients treated with antiretroviral therapy (ART) predict an improved cytolytic T-cell phenotype or better in vivo immune function as measured by immunization responses.HIV-infected patients who started ART during chronic infection and who achieved viral suppression (HIV-RNA400 copies/ml for12 months) were grouped by the presence of strong [stimulation index (SI)10; n = 21] or absent (SI3; n = 18) LP to HIV-core antigen. The two groups were compared for functional immune responses to vaccination with diphtheria-toxoid, tetanus-toxoid and keyhole-limpet-hemocyanin, frequency of circulating naive and memory CD4+ and CD8+ T lymphocytes, maturation phenotype and expression of cytolytic molecules on total and HIV-specific CD8+ T cells, and frequency of memory CD4+ T cells with intracellular HIV-mRNA. Group comparisons were analyzed by non-parametric Mann-Whitney tests. Proportions were estimated by Pearson's chi analysis.There were no differences between the groups in immune responses to vaccination or in the numbers or phenotype of circulating T cells. In a subgroup of HLA-A2+ or B8+ patients, HIV-reactive CD8+ T cells in both groups had similar expression of perforin, granzyme A and T-cell maturation markers (CD27, CD28, CCR7, CD62L). However, patients with SI10 in response to HIVp24 tended to more often have high levels of circulating CD4+ T cells with intracellular HIV-1 mRNA than did patients with SI3.Following long-standing suppression of viral replication on ART, the presence of HIV-1 specific T-helper proliferation responses does not correlate with improved indices of immune phenotype or function but may reflect relatively higher levels of HIV-expression. |
| Databáze: | OpenAIRE |
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