Isolation and purification of lecithin by preparative high-performance liquid chromatography
| Autor: | Charles M. Grill, John V. Amari, Joseph G. Turcotte, Phyllis R. Brown |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Chromatography
food.ingredient Chemistry Organic Chemistry Sulfuric acid General Medicine Biochemistry High-performance liquid chromatography Lecithin Analytical Chemistry chemistry.chemical_compound Countercurrent chromatography food Column chromatography Molybdenum blue Phosphatidylcholines Chromatography Thin Layer Chromatography column Ammonium acetate Chromatography High Pressure Liquid Phospholipids |
| Zdroj: | Journal of Chromatography A. 517:219-228 |
| ISSN: | 0021-9673 |
| Popis: | Mixed-chain, multispecies, egg yolk-derived lecithin was isolated and purified on a silica column with isocratic elution. A method development column (20 x 0.46 cm I.D.) packed with YMC 15-30 microns, 120 A spherical silica and a mobile phase consisting of 5 mM ammonium acetate in acetonitrile-2-propanol-methanol-water (80:13:5:12) was used to separate the lecithin from other phospholipids. The mobile phase conditions for the method development system was adopted for two types of preparative HPLC systems: a Separations Technology SepTech NovaPrep 5000 system with a 20 x 1.93 cm I.D. column and a ST/800A system with a 20 x 5.00 cm I.D. Annular Expansion (A/E) column. The maximum load was 50 microliters of crude solution (2 mg) for the method development column, 0.90 ml (35 mg) for the 20 x 1.93 cm I.D. column and 6.0 ml (240 mg) for the 20 x 5.00 cm I.D. A/E column. The flow-rates were 2, 35 and 235 ml/min, respectively. The fractions collected from the preparative systems were analyzed for purity by analytical-scale high-performance liquid chromatography and by thin-layer chromatography with selective detection with molybdenum blue for phospholipids and detection of all organic compounds by sulfuric acid. Purity of the recovered lecithin was greater than 99%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|