A VARIATION IN THE STRUCTURE OF THE PROTEIN-CODING REGION OF THE HUMAN-P53 GENE
| Autor: | Samarina Op, Natalia Ninkina, Vladimir L. Buchman, Peter M. Chumakov, Georgii P. Georgiev |
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| Předmět: |
Molecular Sequence Data
Restriction Mapping Biology Molecular cloning Cell Line Exon Bacterial Proteins Genetics Humans Amino Acid Sequence Allele Cloning Molecular Enhancer Codon Deoxyribonucleases Type II Site-Specific Gene Base Sequence Nucleic acid sequence Intron Nuclear Proteins General Medicine DNA DNA Neoplasm Phosphoproteins Molecular biology Introns Neoplasm Proteins genomic DNA Blotting Southern Genes Tumor Suppressor Protein p53 Polymorphism Restriction Fragment Length |
| Zdroj: | ResearcherID |
| Popis: | An extensive analysis of genomic DNA preparations from a number of normal and malignant tissues revealed Bgl II site polymorphism of the human p53 gene. Approximately 10% of p53 gene alleles were found to contain an additional Bgl II site localized in a region of intron I. This allelic form of p53 gene was also responsible for p53 protein having altered electrophoretic mobility. Molecular cloning and sequencing of both the alleles of p53 gene revealed a base-pair change in codon 72 causing arginine → proline substitution in the allele with the additional Bgl II site. Both variants of the p53 gene may occur in homozygous state and are therefore functional. |
| Databáze: | OpenAIRE |
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