Performance analysis of the anti-Ki67 antibody clone 30-9 for immunohistochemical staining of breast cancer
Autor: | Shinji Ohno, Shinobu Masuda, Kenichi Togashi, Rie Horii, Hitoshi Tsuda, Futoshi Akiyama, Hironobu Sugita |
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Rok vydání: | 2020 |
Předmět: |
Adult
0301 basic medicine Pathology medicine.medical_specialty Receptor ErbB-2 Clone (cell biology) H&E stain Breast Neoplasms Young Adult 03 medical and health sciences 0302 clinical medicine Breast cancer Image Processing Computer-Assisted Humans Medicine Pharmacology (medical) Radiology Nuclear Medicine and imaging Clinical significance Breast Mastectomy Aged biology business.industry Carcinoma Ductal Breast Significant difference Antibodies Monoclonal General Medicine Middle Aged medicine.disease Immunohistochemistry digestive system diseases Ki-67 Antigen 030104 developmental biology Receptors Estrogen Oncology 030220 oncology & carcinogenesis Morphological analysis biology.protein Female Antibody Receptors Progesterone business |
Zdroj: | Breast Cancer. 27:1058-1064 |
ISSN: | 1880-4233 1340-6868 |
Popis: | Although Ki67 has important clinical relevance in breast cancer, its assessment results vary according to assay due to differences in both analytical and interpretation processes. We aimed to validate the performance of anti-Ki67 antibody clone 30-9 by comparison with clone MIB-1 and to investigate utility of the image analysis system in Ki67 assessment using clinical breast cancer samples. A series of sequential tissue sections was prepared from formalin-fixed paraffin-embedded blocks of surgically resected breast cancer specimens from 50 patients. The tissue sections were stained immunohistochemically with anti-Ki67 antibodies, 30-9 and MIB-1, as well as with hematoxylin and eosin for morphological analysis. We scanned all the stained slides with Ventana iScan HT and selected the Ki67 counting areas based on morphological findings. Three pathologists independently studied images of the counting areas to determine Ki67-positive rates. In addition, the images of 30-9-stained slides were analyzed using the image analysis system, VENTANA Virtuoso. Ki67-positive rates by 30-9 showed a strong correlation with those by MIB-1 for all pathologists (pathologist #1: r = 0.985, pathologist #2: r = 0.987, pathologist #3: r = 0.982). Between 30–9 and MIB-1, there was no significant difference of CV%, showing variabilities of Ki67-positive rates among pathologists. Ki67-positive rates showed a strong correlation between the image analytical values and the pathologist-counted median values (r = 0.952). The performance of 30-9 is equivalent to that of MIB-1 in Ki67 assessment of breast cancer. The image analysis system can substitute for or support visual counting by a pathologist. |
Databáze: | OpenAIRE |
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