Altered cytokine expression of peripheral blood lymphocytes in polymyositis and dermatomyositis
| Autor: | Sándor Sipka, Andrea Ponyi, Péter Antal-Szalmás, Gyula Szegedi, János Hunyadi, Katalin Dankó, Margit Zeher, Andrea Szegedi, Beatrix Irinyi, Magdolna Aleksza, Lajos Gergely |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Adolescent Lymphocyte medicine.medical_treatment T cell Immunology Lymphocyte Activation Polymyositis Dermatomyositis General Biochemistry Genetics and Molecular Biology Immunophenotyping Interferon-gamma Th2 Cells Rheumatology T-Lymphocyte Subsets medicine Humans Immunology and Allergy Cytotoxic T cell Interferon gamma Lymphocyte Count Aged business.industry T-Lymphocytes Helper-Inducer Middle Aged Th1 Cells Flow Cytometry medicine.disease Extended Report Interleukin 10 medicine.anatomical_structure Cytokine Cytokines Female Interleukin-4 business T-Lymphocytes Cytotoxic medicine.drug |
| Zdroj: | Annals of the Rheumatic Diseases. 64:1485-1489 |
| ISSN: | 0003-4967 |
| Popis: | Objective: To investigate the intracellular and soluble cytokine levels and T cell subsets in peripheral blood of patients with active and inactive polymyositis and dermatomyositis. Methods: The frequencies of T and B lymphocytes, T helper (Th), and T cytotoxic (Tc) cells and of interferon γ (IFNγ), interleukin (IL)4, and IL10 expression of CD4+ or CD8+ cells were determined by flow cytometry. The concentrations of soluble cytokines were measured with commercial enzyme linked immunosorbent assays. Results: In active dermatomyositis there was a decreased percentage of T (CD3+) lymphocytes and Tc (CD8+) lymphocytes, decreased IFNγ expression of CD4+ and CD8+ cells, but an increase in B and IL4 producing CD4+ lymphocyte frequencies. These prominent changes disappeared in the inactive stage of the disease. In polymyositis no significant change in these lymphocyte subsets or in intracellular cytokine expression could be detected in either the active or the inactive form. The frequency of IL4+/IFNγ+ Th cells was calculated and a significantly increased Th2/Th1 frequency was found in active dermatomyositis, and a decreased frequency in inactive dermatomyositis, compared with the control population. Conclusions: There appears to be a difference between polymyositis and dermatomyositis in the level of peripheral blood lymphocytes and their intracellular cytokine content. These findings provide further evidence for a difference in the pathogenesis of polymyositis and dermatomyositis. |
| Databáze: | OpenAIRE |
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