Application of SPME/GC-MS To Characterize Metabolites in the Biodesulfurization of Organosulfur Model Compounds in Bitumen

Autor:	Alison M. Jones, Tanya MacPherson, Peter C. K. Lau, Jalal Hawari, Edward Zhou, Matthew J. Grossman, Gesine Wisse, Charles W. Greer, Bruce M. Sankey
Rok vydání:	1998
Předmět:	biology Metabolite Sulfoxide General Chemistry biology.organism_classification Sulfone chemistry.chemical_compound chemistry Dibenzothiophene Environmental Chemistry Organic chemistry Gas chromatography Solid phase extraction Rhodococcus Organosulfur compounds
Zdroj:	Environmental Science & Technology. 32:421-426
ISSN:	1520-5851 0013-936X
DOI:	10.1021/es970356j
Popis:	A combined solid-phase microextraction/GC-MS analytical technique was used to monitor the formation of metabolites in the biodesulfurization of the bitumen model organosulfur compounds, dibenzothiophene (DBT) and the dialkylated derivative 4,6-diethyldibenzothiophene (DEDBT), by Rhodococcus sp. strain ECRD-1. In the case of DBT, the following metabolites were detected: DBT 5-oxide (sulfoxide), DBT 5,5-dioxide (sulfone), dibenz[c,e][1,2]oxathiin 6-oxide (sultine), dibenz[c,e][1,2]oxathiin 6,6-dioxide (sultone), and the end product, 2-hydroxybiphenyl (2-HBP), whereas, with DEDBT, 4,6-DEDBT 5-oxide, 4,6-diethyldibenz[c,e][1,2]oxathiin 6-oxide (sultine), and 2-hydroxy-3,3‘-diethylbiphenyl (HDEBP) as the final product, were identified. A time course study for the formation and disappearance of DBT and DEDBT metabolites was used to construct desulfurization pathways, which in both cases, involved the formation of the corresponding sulfoxides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8c24a7b9b077c04b1adc7d394337460 https://doi.org/10.1021/es970356j Zobrazit plný text záznamu