Mature Purkinje cells in cerebellar tissue cultures: An ultrastructural study

Autor:	Nathan K. Blank, Fredrick J. Seil
Rok vydání:	1982
Předmět:	Dendritic spine General Neuroscience Purkinje cell Parallel fiber Biology Axons Cell biology Mice Microscopy Electron Purkinje Cells Tissue culture Organ Culture Techniques medicine.anatomical_structure Synapses Ultrastructure Extracellular medicine Neuropil Animals Explant culture
Zdroj:	The Journal of Comparative Neurology. 208:169-176
ISSN:	1096-9861 0021-9967
DOI:	10.1002/cne.902080206
Popis:	Mature Purkinje cells in mouse cerebellar tissue cultures were morphologically analyzed by electron microscopy. Explants maintained for 19 to 31 days in vitro contained Purkinje cells that were similar in most respects to those described in vivo except for incomplete arborization of the dendritic trees. Typical features included (1) absence of Purkinje cell perisomatic spines; (2) a paucity of naked Purkinje cell dendritic spines; (3) a 1:1 relationship of Purkinje cell dendritic spines to parallel fiber terminals; and (4) almost complete astroglial investment of Purkinje cell somata and dendrites. Minimal extracellular space was present in the neuropil of the explants and unusual synapses involving Purkinje cells were absent. Atypical features described by some investigators may be a function of retarded development in suboptimal culture conditions and do not represent the limit of tissue culture methodology.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a89ebbff010b028d74e4706f8df9392d https://doi.org/10.1002/cne.902080206 Zobrazit plný text záznamu Plný text