Isolation of human explant derived cardiac stem cells from cryopreserved heart tissue

Autor: Seth Mount, Munir Boodhwani, Darryl R. Davis, Bin Ye, Ross A. Davies, Robyn Jackson, Vincent Chan, Audrey E. Mayfield, Haissam Haddad
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Physiology
Cardiovascular Procedures
Cellular differentiation
Biopsy
Cell
lcsh:Medicine
Blood Pressure
030204 cardiovascular system & hematology
Vascular Medicine
Cryopreservation
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Induced pluripotent stem cell
lcsh:Science
Cells
Cultured
Multidisciplinary
Stem Cells
Cell Differentiation
Heart
Middle Aged
Cardiac Transplantation
3. Good health
Cell biology
Chemistry
medicine.anatomical_structure
Physical Sciences
Female
Stem cell
Cellular Types
Reprogramming
Research Article
Chemical Elements
Pluripotency
Cell Potency
Specimen Preservation
Induced Pluripotent Stem Cells
Surgical and Invasive Medical Procedures
Biology
Research and Analysis Methods
03 medical and health sciences
Paracrine signalling
Cryobiology
Tissue Repair
medicine
Humans
Aged
Cell Proliferation
Transplantation
Myocardium
lcsh:R
Biology and Life Sciences
Cell Biology
Organ Transplantation
Oxygen
030104 developmental biology
Specimen Preparation and Treatment
lcsh:Q
Physiological Processes
Explant culture
Zdroj: PLoS ONE, Vol 12, Iss 4, p e0176000 (2017)
PLoS ONE
ISSN: 1932-6203
Popis: The value of preserving high quality bio specimens for fundamental research is significant as linking cellular and molecular changes to clinical and epidemiological data has fueled many recent advances in medicine. Unfortunately, storage of traditional biospecimens is limited to fixed samples or isolated genetic material. Here, we report the effect of cryopreservation of routine myocardial biopsies on explant derived cardiac stem cell (EDC) culture outcomes. We demonstrate that immediate cryopreservation or delayed cryopreservation after suspension within cardioplegia for 12 hours did not alter EDC yields, proliferative capacity, antigenic phenotype or paracrine signature. Cryopreservation had negligible effects on the ability of EDCs to adopt a cardiac lineage, stimulate new vessel growth, attract circulating angiogenic cells and repair injured myocardium. Finally, cryopreservation did not influence the ability of EDCs to undergo genetic reprogramming into inducible pluripotent stem cells. This study establishes a means of storing cardiac samples as a retrievable live cell source for cardiac repair or disease modeling.
Databáze: OpenAIRE
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