Colon Cancer Associated Transcript-1 (CCAT1) Expression in Adenocarcinoma of the Stomach
| Autor: | Ido Mizrahi, Ronit Grinbaum, Ali O. Gure, Michael Wilschanski, Alexander Stojadinovic, Nahum Beglaibter, Itzhak Avital, Aviram Nissan, Muchamad Adileh, Haggi Mazeh, Vera Pavlov, David Halle |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
Pathology Colon cancer associated transcript 1 Unclassified drug Colorectal cancer Stomach mucosa Laparoscopic surgery Cancer staging Real time polymerase chain reaction Gastroenterology Morbid obesity Gene overexpression Cancer surgery Carcinoma cell line Sleeve gastrectomy Middle aged Quantitative analysis biology Stomach CCAT1 gastrectomy Real-time polymerase chain reaction medicine.anatomical_structure Oncology Long non-coding RNA Adenocarcinoma biomarker Female Human Research Paper Adult medicine.medical_specialty Clinical article Long untranslated RNA Stomach adenocarcinoma Article Gastrectomy Internal medicine medicine Gastric mucosa Human tissue Stomach carcinoma long non-coding RNA Aged Helicobacter pylori business.industry Cancer Very elderly Biomarker biology.organism_classification medicine.disease RNA extraction Gene expression business Gastric cancer Controlled study |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| Popis: | Background: Long non-coding RNAs (lncRNAs) have been shown to have functional roles in cancer biology and are dys-regulated in many tumors. Colon Cancer Associated Transcript -1 (CCAT1) is a lncRNA, previously shown to be significantly up-regulated in colon cancer. The aim of this study is to determine expression levels of CCAT1 in gastric carcinoma (GC). Methods: Tissue samples were obtained from patients undergoing resection for gastric carcinoma (n=19). For each patient, tumor tissue and normal appearing gastric mucosa were taken. Normal gastric tissues obtained from morbidly obese patients, undergoing laparoscopic sleeve gastrectomy served as normal controls (n=19). A human gastric carcinoma cell line (AGS) served as positive control. RNA was extracted from all tissue samples and CCAT1 expression was analyzed using quantitative real time-PCR (qRT-PCR). Results: Low expression of CCAT1 was identified in normal gastric mucosa samples obtained from morbidly obese patients [mean Relative Quantity (RQ) = 1.95±0.4]. AGS human gastric carcinoma cell line showed an elevated level of CCAT1 expression (RQ=8.02). Expression levels of CCAT1 were approximately 10.8 fold higher in GC samples than in samples taken from the negative control group (RQ=21.1±5 vs. RQ=1.95±0.4, respectively, p |
| Databáze: | OpenAIRE |
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