Fully automated microchip system for the detection of quantal exocytosis from single and small ensembles of cells
| Autor: | Christer Spegel, Arto Heiskanen, Simon Pedersen, Rafael J. Taboryski, Jenny Emnéus, Tautgirdas Ruzgas |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Patch-Clamp Techniques
Time Factors Surface Properties Dopamine Potassium Biomedical Engineering Analytical chemistry chemistry.chemical_element Bioengineering PC12 Cells Sensitivity and Specificity Biochemistry Exocytosis Cell membrane Catecholamines Electrochemistry medicine Animals 3-Mercaptopropionic Acid Chemistry Reproducibility of Results Depolarization General Chemistry Cells Immobilized Microfluidic Analytical Techniques Microarray Analysis Rats Full width at half maximum medicine.anatomical_structure Fully automated Electrode Calcium Microelectrodes Sensitivity (electronics) |
| Zdroj: | Lab Chip. 8:323-329 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/b715107a |
| Popis: | A lab-on-a-chip device that enables positioning of single or small ensembles of cells on an aperture in close proximity to a mercaptopropionic acid (MPA) modified sensing electrode has been developed and characterized. The microchip was used for the detection of Ca(2+)-dependent quantal catecholamine exocytosis from single as well as small assemblies of rat pheochromocytoma (PC12) cells. The frequency of events increased considerably upon depolarization of the PC12 cell membrane using a high extracelluar concentration of potassium. The number of recorded events could be correlated with the number of cells immobilized on the electrode. Quantal characteristics, such as the number of released molecules per recorded event, are equivalent to data obtained using conventional carbon fiber microelectrodes. The detection sensitivity of the device allows for the detection of less than 10 000 dopamine molecules in a quantal release. The distribution of peak rise-time and full width at half maximum was constant during measurement periods of several minutes demonstrating the stability of the MPA modified surface. |
| Databáze: | OpenAIRE |
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