TGF-beta enhances osteoclast differentiation in hematopoietic cell cultures stimulated with RANKL and M-CSF
| Autor: | Jeffrey W. Horn, Tina Fuson, Cindy L. Gatlin, Rachelle J. Sells Galvin |
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| Rok vydání: | 1999 |
| Předmět: |
Male
medicine.medical_specialty Stromal cell Cellular differentiation Biophysics Osteoclasts Bone Marrow Cells Biochemistry Cell Line Mice Multinucleate Bacterial Proteins Osteoclast Transforming Growth Factor beta Internal medicine medicine Animals Lymphocytes Calcitonin receptor Bone Resorption Molecular Biology Cells Cultured Cell Size Cell Nucleus Mice Inbred BALB C Membrane Glycoproteins biology Receptor Activator of Nuclear Factor-kappa B Chemistry Macrophage Colony-Stimulating Factor RANK Ligand Skull RNA-Binding Proteins Cell Differentiation Cell Biology Receptors Calcitonin Hematopoietic Stem Cells Coculture Techniques Cell biology Haematopoiesis Endocrinology medicine.anatomical_structure Cell culture RANKL biology.protein Cattle Carrier Proteins Spleen Transcription Factors |
| Zdroj: | Biochemical and biophysical research communications. 265(1) |
| ISSN: | 0006-291X |
| Popis: | TGF-beta has been shown to inhibit and stimulate osteoclastogenesis. The purpose of this study was to evaluate the effects of TGF-beta in hematopoietic cell cultures stimulated with RANKL and M-CSF. In cocultures of hematopoietic cells and BALC cells (a calvarial-derived cell line), TGF-beta inhibited tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cell formation. In contrast, TGF-beta enhanced TRAP-positive multinucleated cell formation up to 10-fold in hematopoietic cell cultures containing few osteoblastic/stromal cells. Likewise, TGF-beta increased the number of calcitonin receptor (CTR)-positive multinucleated and mononucleated cells in a concentration-dependent manner. An increase in cell size and multinuclearity was also observed in the presence of TGF-beta. The stimulatory effects of TGF-beta were dependent on the presence of M-CSF and RANKL. When differentiated on bovine cortical bone slices, these cells formed resorption lacunae. These results suggest that TGF-beta has a direct stimulatory effect on osteoclastogenesis in hematopoietic cells treated with RANKL and M-CSF. |
| Databáze: | OpenAIRE |
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