Tenascin and Cytokines in Tear Fluid After Photorefractive Keratectomy
Autor: | Timo T Tervo, Minna H Vesaluoma |
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Rok vydání: | 1998 |
Předmět: |
Adult
Male medicine.medical_specialty Time Factors Adolescent VEGF receptors medicine.medical_treatment Urology Tenascin Photorefractive Keratectomy Cornea 03 medical and health sciences 0302 clinical medicine Basic knowledge Preoperative level Humans Medicine Growth Substances 030304 developmental biology Immunoassay Wound Healing 0303 health sciences biology business.industry Mean age Middle Aged Photorefractive keratectomy Refractive Surgical Procedures 3. Good health Surgery Ophthalmology Tears 030221 ophthalmology & optometry biology.protein Female Lasers Excimer business |
Zdroj: | Journal of Refractive Surgery. 14:447-454 |
ISSN: | 1081-597X |
DOI: | 10.3928/1081-597x-19980701-11 |
Popis: | BACKGROUND: Basic knowledge of the substances involved in wound healing after photorefractive keratectomy (PRK) is essential for development of pharmacological intervention. We present preoperative and postoperative analysis of tear fluid extracellular matrix proteins and cytokines after PRK. METHODS: Tear fluid samples from 70 patients (72 eyes) who had PRK (38 women and 32 men, mean age 31.5 yr) were studied. Samples from 18 patients (18 eyes) were analyzed in two different studies. RESULTS: Mean preoperative tear fluid flow in the collection capillary (volume divided by tear collection time) varied from 4.5 to 22.5 µ?/min in five series of patients. It increased significantly during the first two postoperative days (range of means, 55.5 to 88.8 µ? min, ? < 0.01), and decreased to the preoperative level by day 7 (range of means, 9.7 to 18.2 µ?/min). The tenascin and cytokine release rates increased significantly during the first two days after PRK and returned to the preoperative level by day 7. Mean ± standard error for tenascin: day 0 (5.2 ±1.9 ng/min); day 2 (22.7 ±6.1 ng/min; p=0.02). Mean ± standard error for HGF: day 0 (3.2 ± 0.7 pg/min); day 1 (22.8 ± 4.2 pg/min; p=0.0003). Mean ± standard error for TGF-ßl: day 0 (63.3 ± 19.6 pg/min); days 1-2 (826.2 ± 253.7 pg/min; p=0.001). Mean ± standard error for VEGF: day 0 (166.0 ± 29.6 pg/min); days 1-2 (824.4 ± 165.1 pg/min; p=0.0007). Mean ± standard error for PDGF-BB: day 0 (0.42 ± 0.19 pg/min); day 2 (27.6 ± 5.8 pg/min; p=0.0000). Mean ± standard error for TNF-a: day 0 (9.5 ± 2.6 pg/min); day 2 (28.6 ± 5.9 pg/min; p=0.003). Excluding PDGF-BB, all substances studied were present in normal human tear fluid. PDGF-BB was present in only 17% of the preoperative samples. CONCLUSION: Corneal wounding induces an increased release of several growth modulating cytokines which may be involved in healing processes. [J Refract Surg 1998;14:447-4541 |
Databáze: | OpenAIRE |
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