Differential IL-12 responsiveness of T cells but not of NK cells from tumor-bearing mice in IL-12-responsive versus -unresponsive tumor models
| Autor: | Chigusa Nakajima, Toshiyuki Hamaoka, Ping Gao, Masayuki Iwasaki, Yasuhiro Uekusa, Rishani Wijesuriya, Wen Gong Yu, Yi Fu Yang, Hiromi Fujiwara |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male medicine.medical_specialty Time Factors T-Lymphocytes Immunology Population Enzyme-Linked Immunosorbent Assay CD8-Positive T-Lymphocytes Biology Interferon-gamma Mice chemistry.chemical_compound Interleukin 21 Antigen Internal medicine Tumor Cells Cultured medicine Animals Antigens Ly Immunology and Allergy Cytotoxic T cell Lectins C-Type RNA Messenger Antigens education Interleukin 3 Mice Inbred BALB C Mice Inbred C3H education.field_of_study Receptors Interleukin-12 Proteins Neoplasms Experimental Receptors Interleukin General Medicine T lymphocyte Meth Interleukin-12 Killer Cells Natural Mice Inbred C57BL Endocrinology chemistry Antigens Surface Interleukin 12 Female Tumor Escape Spleen NK Cell Lectin-Like Receptor Subfamily B |
| Zdroj: | International Immunology. 12:701-709 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/12.5.701 |
| Popis: | While IL-12 administration induces tumor regression through stimulating T cells in tumor-bearing mice, this IL-12 effect is observed in some but not all tumor models. The present study aimed to compare IL-12 responsiveness of T cells from tumor-bearing mice in IL-12-responsive (CSA1M and OV-HM) and -unresponsive (Meth A) tumor models. Tumor regression in IL-12-responsive tumor models required the participation of T cells, but not of NK1.1(+) cells. Because a NK1.1(+) cell population was the major producer of IFN-gamma, comparable levels of IFN-gamma production were induced in IL-12-responsive and -unresponsive tumor-bearing mice. This indicates that the amount of IFN-gamma produced in tumor-bearing individuals does not correlate with the anti-tumor efficacy of IL-12. In contrast, IL-12 responsiveness of T cells differed between the responsive and unresponsive models: purified T cells from CSA1M/OV-HM-bearing or Meth A-bearing mice exhibited high or low IL-12 responsiveness respectively, when evaluated by the amounts of IFN-gamma produced in response to IL-12. T cells from CSA1M- or OV-HM-bearing but not from Meth A-bearing mice exhibited enhanced levels of mRNA for the IL-12 receptor (IL-12R). These results indicate that a fundamental difference exists in IL-12 responsiveness of T cells between IL-12-responsive and -unresponsive tumor models, and that such a difference is associated with the expression of IL-12R on T cells. |
| Databáze: | OpenAIRE |
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