New class of poly(vinyl alcohol) polymers as column-chromatography stationary phases for Candida rugosa lipase isoforms separation
| Autor: | Marisa Delbò, Simonetta Soro, Alessandro Pala, Giancarlo Ortaggi, Enrico Cernia, Lucia Battinelli |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Vinyl alcohol
Chromatography biology Polymers Hydrophilic interaction chromatography Organic Chemistry Triacylglycerol lipase Lipase General Medicine Biochemistry Polyvinyl alcohol Analytical Chemistry Candida rugosa Electrophoresis chemistry.chemical_compound Column chromatography chemistry Polyvinyl Alcohol Microscopy Electron Scanning biology.protein Organic chemistry Electrophoresis Polyacrylamide Gel Candida Chromatography Liquid |
| Zdroj: | Journal of Chromatography A. 753:47-55 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(96)00482-7 |
| Popis: | The preparation of new stationary phases of cross-linked polyvinyl alcohol esterified with various linear fatty acids is described. The physico-chemical properties of these polymers are reported, including electron microscopy and swelling measurements. Batch adsorption experiments were performed in order to characterize the basic separative properties of these phases. Cross-linked polyvinyl alcohol esterified with dodecanoic acid was used for hydrophobic interaction chromatography of a commercial crude preparation of Candida rugosa lipase. Characterization of the purified fractions was carried out via native electrophoresis and sodium dodecyl sulfate-polyacrylamide electrophoresis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect