Parasitoid wasp venom vesicles (venosomes) enter Drosophila melanogaster lamellocytes through a flotillin/lipid raft-dependent endocytic pathway
| Autor: | Marylène Poirié, Jean-Luc Gatti, Bin Wan |
|---|---|
| Přispěvatelé: | Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Department of Plant Health and Environment (SPE) from the French National Research Institute for Agriculture, Food and Environment (INRAE), DROPSA program, ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), ANR-15-IDEX-0001,UCA JEDI,Idex UCA JEDI(2015), European Project, Université Nice Sophia Antipolis (... - 2019) (UNS) |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
parasitoid wasps Endosome media_common.quotation_subject [SDV]Life Sciences [q-bio] Immunology Endocytic cycle Infectious and parasitic diseases RC109-216 Biology Endocytosis confocal microscopy Microbiology Clathrin rafts 03 medical and health sciences 0302 clinical medicine lysosomes venosomes Internalization Lipid raft 030304 developmental biology media_common 0303 health sciences Lamellocytes Pinocytosis Endoplasmic reticulum 3. Good health Cell biology Infectious Diseases Drosophila melanogaster endosomes biology.protein Parasitology 030217 neurology & neurosurgery |
| Zdroj: | Virulence Virulence, 2020, 11 (1), pp.1512-1521. ⟨10.1080/21505594.2020.1838116⟩ Virulence, Taylor & Francis, 2020, 11 (1), pp.1512-1521. ⟨10.1080/21505594.2020.1838116⟩ Virulence, Vol 11, Iss 1, Pp 1512-1521 (2020) |
| ISSN: | 2150-5594 2150-5608 |
| DOI: | 10.6084/m9.figshare.13172677 |
| Popis: | Venosomes are extracellular vesicles found in the venom of Leptopilina endoparasitoids wasps, which transport and target virulence factors to impair the parasitoid egg encapsulation by the lamellocytes of their Drosophila melanogaster host larva. Using the co-immunolocalization of fluorescent L. boulardi venosomes and one of the putative-transported virulence factors, LbGAP, with known markers of cellular endocytosis, we show that venosomes endocytosis by lamellocytes is not a process dependent on clathrin or macropinocytosis and internalization seems to bypass the early endosomal compartment Rab5. After internalization, LbGAP colocalizes strongly with flotillin-1 and the GPI-anchored protein Atilla/L1 (a lamellocyte surface marker) suggesting that entry occurs via a flotillin/lipid raft-dependent pathway. Once internalized, venosomes reach all intracellular compartments, including late and recycling endosomes, lysosomes, and the endoplasmic reticulum network. Venosomes therefore enter their target cells by a specific mechanism and the virulence factors are widely distributed in the lamellocytes’ compartments to impair their functions. |
| Databáze: | OpenAIRE |
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