Transglutaminase binding fusion protein linked to SLPI reduced corneal inflammation and neovascularization

Autor: Juan Pablo Salica, Diego Guerrieri, H. Eduardo Chuluyan, Paulo Cesar Maffia, Juan E Gallo, J. O. Croxatto
Jazyk: angličtina
Předmět:
Male
Vascular Endothelial Growth Factor A
Pathology
Tissue transglutaminase
Administration
Topical
Cell Count
Corneal inflammation
ANGIOGENESIS
Rats
Sprague-Dawley
Neovascularization
Cementoin
Re-Epithelialization
Cornea
Secretory Leukocyte Peptidase Inhibitor
CORNEAL NEOVASCULARIZATION
Fluorescent Antibody Technique
Indirect
Alkali injury
Corneal epithelium
biology
Epithelium
Corneal
purl.org/becyt/ford/3.1 [https]
General Medicine
Medicina Básica
Eye Burns
medicine.anatomical_structure
purl.org/becyt/ford/3 [https]
medicine.symptom
Research Article
Farmacología y Farmacia
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
ALKALI INJURY
Recombinant Fusion Proteins
NFkB
GTP-Binding Proteins
Burns
Chemical
medicine
Animals
Corneal Neovascularization
Protein Glutamine gamma Glutamyltransferase 2
Keratitis
Transglutaminases
SLPI
business.industry
Histology
medicine.disease
Transglutaminase
eye diseases
Rats
CORNEAL INFLAMMATION
Disease Models
Animal
Ophthalmology
CEMENTOIN
TRANSGLUTAMINASE
Corneal neovascularization
biology.protein
Angiogenesis
sense organs
business
Zdroj: CONICET Digital (CONICET)
Consejo Nacional de Investigaciones Científicas y Técnicas
instacron:CONICET
BMC Ophthalmology
ISSN: 1471-2415
DOI: 10.1186/1471-2415-15-12
Popis: Background: To study the effect of topical administration of a fusion protein (PF-MC) made up of N-terminal portion of the protease inhibitor Trappin-2 (which is a substrate of transglutaminasa-2) and SLPI (protein with anti-inflammatory, anti-bacterial and anti-viral ability), in an animal model of corneal inflammation and angiogenesis. Methods: An alkali injury was produced with a filter paper of 3 mm with 1 N NaOH during 40 seconds on the right cornea of 36 male Sprague Dawley rats, under general anesthesia. Animals were divided into three groups according to treatment. Group 1 was treated with 10 ul of PF-MC (200 ug/ml; n = 12), Group 2, with 10 ul of SLPI (200 ug/ml; n = 12) and Group 3 was treated with buffer (10 ul; n = 12) topically administered four times a day for up to 7 days. Half of the animals were sacrificed at day 3 before making a re-epithelialization time analysis with fluorescein staining at 18 and 24 hours. In the remaining animals corneal opacity was studied and digital photographs were taken at day 7 before doing euthanasia. Eyes were processed for histology and immunofluorescence. Results: Corneal ulcerated area was significantly lower in PF-MC treated animals compared to SLPI and buffer-treated animals at 18 hours and 24 hours postinjury. A clear cornea and fundus red reflex was only found among PF-MC treated animals. Histological analysis revealed a stratified corneal epithelium with at least three layers in all PF-MC animals at day 7. In this group there was a reduced number of PMNs in the corneal stroma at 3 and 7 days of follow-up. Besides, corneal neovascularization was much more extended in SLPI and Buffer animals than in animals treated with PF-MC. Conclusions: The binding of SLPI with Cementoin to transglutaminase seems to be an effective strategy to treat corneal inflammation and angiogenesis. Fil: Salica, Juan Pablo. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina. Hospital Universitario Austral. Departamento de Oftalmología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Maffia, Paulo Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Croxatto, Juan Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fundación Oftalmología Argentina "J. Malbrán"; Argentina Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos; Argentina. Universidad de Buenos Aires. Facultad de Medicina; Argentina Fil: Gallo, Juan Eduardo Maria. Universidad Austral. Facultad de Ciencias Biomedicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Hospital Universitario Austral. Departamento de Oftalmología; Argentina
Databáze: OpenAIRE
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