Photography Coupled with Self-Propagating Chemical Cascades: Differentiation and Quantitation of G- and V‑Nerve Agent Mimics via Chromaticity
| Autor: | Pedro Metola, Leilani Smith, Alexander A. Boulgakov, Xiaolong Sun, Eric V. Anslyn, Edward M. Marcotte |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Analyte
010405 organic chemistry Chemistry Calibration curve General Chemical Engineering Photography General Chemistry Color space 010402 general chemistry 01 natural sciences Fluorescence 0104 chemical sciences medicine Diisopropyl fluorophosphate Chromaticity Biological system QD1-999 Research Article medicine.drug Nerve agent |
| Zdroj: | ACS Central Science, Vol 4, Iss 7, Pp 854-861 (2018) ACS Central Science |
| ISSN: | 2374-7951 |
| Popis: | Photography was employed for the quantitation and differentiation of G- and V-series nerve agent mimics with the use of self-propagating cascades. Fluoride anion and thiols, released from a G-nerve agent mimic (i.e., diisopropyl fluorophosphate) and a V-nerve agent mimic (i.e., demeton-S-methyl), respectively, were used to initiate self-propagating cascades that amplify fluorescence signals exponentially in a ratiometric manner. A homemade LEGO dark-box, a cell phone, and 96-well plates were employed to collect photographs of the fluorescence response to the analytes. The photographic images were digitally processed in the 1931 xyY color space using a watershed and morphological erosion algorithm to generate chromaticity vs concentration calibration curves. We show that the two different amplification routines are selective for their analyte class and thus successfully discriminated the G- and V-series nerve agent mimics. Further, accurate concentrations of the analytes are determined using the chromaticity and LEGO approach given herein, thus demonstrating a simple and on-site constructible/portable device for use in the field. Fluorescence chromaticity, referring to the CIE 1930 color space, coupled with chemical self-propagating cascades, differentiate and quantitate nerve agents mimics, demonstrating a portable device. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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