Long non-coding RNA PVT1 can regulate the proliferation and inflammatory responses of rheumatoid arthritis fibroblast-like synoviocytes by targeting microRNA-145-5p
| Autor: | Jiajun Tang, Shiyu Yi, Yi Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Fibroblast-like synoviocyte Adult Male Cancer Research Gene Expression Apoptosis Flow cytometry Proinflammatory cytokine Arthritis Rheumatoid 03 medical and health sciences 0302 clinical medicine microRNA medicine Humans Molecular Targeted Therapy Cells Cultured Aged Cell Proliferation Inflammation Gene knockdown medicine.diagnostic_test Cell growth Chemistry Tumor Necrosis Factor-alpha Interleukin Cell Biology Middle Aged Synoviocytes MicroRNAs 030104 developmental biology 030220 oncology & carcinogenesis Cancer research Cytokines Female RNA Long Noncoding Inflammation Mediators |
| Zdroj: | Human cell. 33(4) |
| ISSN: | 1749-0774 |
| Popis: | Long non-coding RNAs (lncRNAs) function in rheumatoid arthritis (RA). The present work was designed to explore the roles of lncRNA PVT1 in RA and the related mechanism. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to determine mRNA level. The binding sites between PVT1 and miR-145-5p were verified by a dual-luciferase reporter assay. Furthermore, RA-FLSs were treated with TNF-α to establish the RA model. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) and 5-ethynyl-2'-deoxyuridine (EdU) assays were performed to detect cell proliferation. Flow cytometry and TUNEL assays were performed to detect cell apoptosis. Enzyme-linked immunosorbent assay (ELISA) was used to determine levels of inflammatory cytokines. PVT1 was significantly increased and miR-145-5p was decreased in synovial tissues of RA patients. miR-145-5p is a target miRNA of PVT1, and the levels of PVT1 and miR-145-5p in synovial tissues of RA patients were negatively correlated. In RA-FLSs, tumour necrosis factor-α (TNF-α) led to increased PVT1 levels and decreased miR-145-5p levels. Knockdown of PVT1 inhibited TNF-α-induced RA-FLS over-proliferation and reversed TNF-α-induced RA-FLS apoptosis reduction. Moreover, knockdown of PVT1 inhibited TNF-α-induced production of interleukin (IL)-1β and IL-6 and the activation of NF-κB through miR-145-5p. PVT1 can regulate apoptosis and inflammatory responses in RA-FLSs by targeting miR-145-5p. |
| Databáze: | OpenAIRE |
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