Flow cytometry and IG/TCR quantitative PCR for minimal residual disease quantitation in acute lymphoblastic leukemia: a French multicenter prospective study on behalf of the FRALLE, EORTC and GRAALL
| Autor: | Yves Bertrand, S Marty-Grès, Jean Tkaczuk, Hervé Dombret, Nelly Robillard, Francine Garnache-Ottou, F. Huguet, André Baruchel, Isabelle Arnoux, Chantal Fossat, Chantal Brouzes, Hélène Cavé, Emmanuelle Clappier, Kheira Beldjord, Elisabeth Macintyre, Marie-Laure Boulland, Thierry Fest, MC Béné, Norbert Ifrah, Marie-Christine Jacob, Emilienne Kuhlein, Eric Delabesse, Adriana Plesa, Vahid Asnafi, Mikael Roussel, Richard Garand |
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| Rok vydání: | 2012 |
| Předmět: |
Oncology
Adult Male Cancer Research medicine.medical_specialty Neoplasm Residual Real-Time Polymerase Chain Reaction Sensitivity and Specificity Flow cytometry hemic and lymphatic diseases Internal medicine Medicine Humans Prospective Studies Prospective cohort study Child Survival rate Gene Rearrangement medicine.diagnostic_test Genes Immunoglobulin business.industry T-cell receptor Infant Hematology Gene rearrangement DNA Neoplasm Precursor Cell Lymphoblastic Leukemia-Lymphoma Flow Cytometry Prognosis Minimal residual disease Survival Rate Genes T-Cell Receptor medicine.anatomical_structure Real-time polymerase chain reaction Child Preschool Immunology Female Bone marrow business Follow-Up Studies |
| Zdroj: | Leukemia. 27(2) |
| ISSN: | 1476-5551 |
| Popis: | Minimal residual disease (MRD) quantification is widely used for therapeutic stratification in pediatric acute lymphoblastic leukemia (ALL). A robust, reproducible, sensitivity of at least 0.01% has been achieved for IG/TCR clonal rearrangements using allele-specific quantitative PCR (IG/TCR-QPCR) within the EuroMRD consortium. Whether multiparameter flow cytometry (MFC) can reach such inter-center performance in ALL MRD monitoring remains unclear. In a multicenter study, MRD was measured prospectively on 598 follow-up bone marrow samples from 102 high-risk children and 136 adult ALL patients, using IG/TCR-QPCR and 4/5 color MFC. At diagnosis, all 238 patients (100%) had at least one suitable MRD marker with 0.01% sensitivity, including 205/238 samples (86%) by using IG/TCR-QPCR and 223/238 samples (94%) by using MFC. QPCR and MFC were evaluable in 495/598 (83%) samples. Qualitative results ( |
| Databáze: | OpenAIRE |
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