Serum antibodies anti-H. pylori and anti-CagA: A comparison between four different assays
| Autor: | Filippo Navaglia, Francesco Di Mario, L Brigato, Mario Plebani, Eliana Greco, A Toma, Maria Grazia Piva, Daniela Basso, Anna-Lisa Stefani, Carlo Federico Zambon |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Microbiology (medical) Adolescent Gastrointestinal Diseases Clinical Biochemistry Enzyme-Linked Immunosorbent Assay Helicobacter Infections law.invention Bacterial Proteins law medicine Humans Immunology and Allergy CagA Child Polymerase chain reaction Aged Antigens Bacterial Helicobacter pylori biology Biochemistry (medical) Public Health Environmental and Occupational Health Histology Original Articles Hematology Middle Aged bacterial infections and mycoses medicine.disease Antibodies Bacterial Virology Medical Laboratory Technology Child Preschool Data Interpretation Statistical Peptic ulcer biology.protein Female Antibody Gastritis medicine.symptom |
| Zdroj: | Journal of Clinical Laboratory Analysis. 13:194-198 |
| ISSN: | 1098-2825 0887-8013 |
| DOI: | 10.1002/(sici)1098-2825(1999)13:4<194::aid-jcla9>3.0.co;2-e |
| Popis: | The authors compare efficacy of two ELISA assays (one supplied by DIAMEDIX [Delta Biological s.r.l.], and the other by RADIM [RADIM I]) in detecting total anti‐H. pylori antibodies, and of two further ELISA methods (one supplied by EUROSPITAL [Helori CTX IgG] and the other by RADIM [RADIM 2]) in identifying anti‐CagA antibodies, using sera from 69 controls (20 adults and 49 children) and from 96 patients, obtained before endoscopy. Seventy‐three of the patients had H. pylori infection, while the remaining 23 were H. pylori negative (histology and polymerase chain reaction [PCR]). Fifty‐two of the H. pylori positive patients, had cagA‐positive strain infection, identified by PCR. The DIAMEDIX assay was found to be more sensitive (92%) than RADIM 1 (79%) in identifying H. pylori positive patients, irrespective of the infecting strain. On the other hand, the DIAMEDIX assay was less specific than RADIM 1 for H. pylori‐negative patients (43% vs. 83%). However, when patients already treated for H. pylori infection were excluded from the group of H. pylori‐negative patients, the DIAMEDIX assay had a specificity of 89%. In identifying anti‐CagA antibodies, the kit supplied by RADIM (RADIM 2) had a sensitivity of 90% and a specificity of 94%, whereas that supplied by EUROSPITAL had a sensitivity of 100% and a specificity of 76%. The performances of the two methods in the identification of anti‐CagA antibodies were found to be similar. The authors conclude that, in view of its high sensitivity, the DIAMEDIX assay may be useful in screening for H. pylori infection. J. Clin. Lab. Anal. 13:194–198, 1999. © 1999 Wiley‐Liss, Inc. |
| Databáze: | OpenAIRE |
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