B-Cell Depletion Improves Islet Allograft Survival with Anti-CD45RB
| Autor: | James F. Markmann, Gaoping Zhao, R. Stott, Matthew R O’Connor, Paolo Fiorina, Kyri Dunussi, Heidi Yeh, Kang Mi Lee, Lingling Wei, James I. Kim, Shaoping Deng, J. Soohoo |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Islets of Langerhans Transplantation Biomedical Engineering lcsh:Medicine Mice Transgenic Biology Article Diabetes Mellitus Experimental Mice Allograft survival Animals B-Lymphocytes Mice Inbred BALB C Transplantation geography geography.geographical_feature_category Cardiac allograft Graft Survival lcsh:R Cell Biology Allografts Islet Mice transgenic Mice Inbred C57BL Disease Models Animal Tolerance induction surgical procedures operative B cell depletion Immunology Leukocyte Common Antigens Transplantation Tolerance Graft survival |
| Zdroj: | Cell Transplantation, Vol 23 (2014) |
| ISSN: | 1555-3892 0963-6897 |
| DOI: | 10.3727/096368912x658962 |
| Popis: | A short course of anti-CD45RB leads to long-term islet allograft survival and donor-specific tolerance in approximately half of immunocompetent mice. We have previously demonstrated that anti-CD45RB antibody-mediated tolerance requires B-cells for cardiac allograft survival. We therefore asked whether B-cells were also required for anti-CD45RB antibody-mediated survival of islets. Unexpectedly, we found that nearly 100% of islet allografts survive long term in B-cell-deficient mice. Similarly, B-cell depletion by anti-CD22/cal augmented anti-CD45RB-mediated tolerance when administered pretransplant, although it had no effect on tolerance induction when administered posttransplant. Our results demonstrate that the role of B-cells in promoting tolerance with anti-CD45RB is graft specific, promoting tolerance in cardiac grafts but resisting tolerance in islet transplantation. These findings may help elucidate the varied action of B-cells in promoting tolerance versus rejection. |
| Databáze: | OpenAIRE |
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