Validation of the long‐term assessment of hypothalamic‐pituitary‐adrenal activity in rats using hair corticosterone as a biomarker
| Autor: | Jordi Pastor-Ciurana, Javier Carrasco, Fabrizio Scorrano, Alicia Rami-Bastante, Xavier Belda, Antonio Armario, Maria Laura Bacci |
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| Přispěvatelé: | Scorrano F, Carrasco J, Pastor-Ciurana J, Belda X, Rami-Bastante A, Bacci ML, Armario A |
| Rok vydání: | 2014 |
| Předmět: |
Male
Hypothalamo-Hypophyseal System medicine.medical_specialty chronic unpredictable stre medicine.medical_treatment Anti-Inflammatory Agents Radioimmunoassay Pituitary-Adrenal System Chronic stressor Biochemistry Rats Sprague-Dawley Hpa activity chemistry.chemical_compound Corticosterone Internal medicine Male rats Genetics medicine Animals Chronic stress Glucocorticoids Molecular Biology Total plasma glucocorticoids business.industry Adrenalectomy Chronic immobilization Rats ACTH chronic immobilization Endocrinology chemistry Rat Biomarker (medicine) Female Chronic unpredictable stress business Biomarkers Stress Psychological Hair Biotechnology |
| Zdroj: | Dipòsit Digital de Documents de la UAB Universitat Autònoma de Barcelona |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.14-254474 |
| Popis: | The evaluation of chronic activity of the hypothalamic-pituitary-adrenal (HPA) axis is critical for determining the impact of chronic stressful situations. However, current methods have important limitations. The potential use of hair glucocorticoids as a noninvasive retrospective biomarker of long-term HPA activity is gaining acceptance in humans and wild animals. However, there is no study examining hair corticosterone (HC) in laboratory animals. The present study validates a method for measuring HC in rats and demonstrates that it properly reflects chronic HPA activity. The HC concentration was similar in male and female rats, despite higher total plasma corticosterone levels in females, tentatively suggesting that it reflects free rather than total plasma corticosterone. Exposure of male rats to 2 different chronic stress protocols (chronic immobilization and chronic unpredictable stress) resulted in similarly higher HC levels compared to controls (1.8-fold). HC also increased after a mild chronic stressor (30 min daily restraint). Chronic administration of 2 different doses of a long-acting ACTH preparation dramatically increased HC (3.1- and 21.5-fold, respectively), demonstrating that a ceiling effect in HC accumulation is unlikely under other more natural conditions. Finally, adrenalectomy significantly reduced HC. In conclusion, HC measurement in rats appears appropriate to evaluate integrated chronic changes in circulating corticosterone. |
| Databáze: | OpenAIRE |
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