BONEFILL® block as alternative for bone substitute: a toxicological evaluation
| Autor: | Sybele Saska, Fernanda Coelho, Raquel M. Scarel-Caminaga, Karine Melchior, Ticiana Sidorenko de Oliveira Capote |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hydroxyapatite lcsh:RS1-441 02 engineering and technology medicine.disease_cause lcsh:Pharmacy and materia medica 03 medical and health sciences chemistry.chemical_compound Biomaterials/evaluation medicine Viability assay Bone regeneration Cytotoxicity Chemistry technology industry and agriculture BONEFILL®/toxicity 021001 nanoscience & nanotechnology Molecular biology Comet assay PLGA 030104 developmental biology Micronucleus test 0210 nano-technology Micronucleus Genotoxicity |
| Zdroj: | Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 2 (2018); e17438 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 2 (2018); e17438 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 2 (2018); e17438 Brazilian Journal of Pharmaceutical Sciences Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Pharmaceutical Sciences, Vol 54, Iss 2 (2018) Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1984-8250 2175-9790 |
| Popis: | Made available in DSpace on 2018-12-11T17:37:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01. Added 1 bitstream(s) on 2021-07-15T15:22:29Z : No. of bitstreams: 1 S1984-82502018000200611.pdf: 877438 bytes, checksum: 189304a72071d411a1c0132e1941c106 (MD5) Bone substitutes based on hydroxyapatite (HA) and Bonefill® (BO-inorganic bovine bone) associated with poly(lactic-co-glycolic acid) (PLGA) (HA/PLGA and BO/PLGA) were evaluated concerning cytotoxicity, genotoxicity and mutagenicity as potential candidates for bone repair. The materials were developed and provided by Bionnovation Biomedical Products Ltda. Eluates from these bone substitutes were prepared for toxicity evaluations using eukaryotic cell cultures. HA/PLGA was used as a comparison for Bonefill®. Cell viability was evaluated by XTT assay and surviving fraction was calculated for clonogenic survival. Additionally, tail moment was used to assess genotoxicity (comet assay). The frequencies of binucleated cells with micronucleus (FBMN), micronucleus (FMN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) were analysed by cytokinesis-block micronucleus assay (CBMN assay). Results showed no statistical difference in cell viability compared with negative control (NC) The eluates did not promote delayed cytotoxicity whereas the surviving fraction rate for cultured cells was similar to NC. Furthermore, no genotoxicity or mutagenicity effects were observed for cultured cells with the Bonefill/PLGA and HA/PLGA eluates. In conclusion, the negative cytotoxicity, genotoxicity and mutagenicity results indicate that these bone substitutes presented interesting preliminary results as potential biomaterials for bone repair. São Paulo State University (UNESP) School of Pharmaceutical Sciences São Paulo State University (UNESP) School of Dentistry São Paulo State University (UNESP) School of Pharmaceutical Sciences São Paulo State University (UNESP) School of Dentistry |
| Databáze: | OpenAIRE |
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