Effect of Metabolite Extract of Streptomyces hygroscopicus subsp. hygroscopicus on Plasmodium falciparum 3D7 in Vitro
| Autor: | Agustina Tri Endharti, Annisa Alkarimah, Wahyudha Ngatiril Lady, Loeki Enggar Fitri, Alfian Wika Cahyono, Rivo Yudhinata Brian Nugraha |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
Metabolite 01 natural sciences Streptomyces lcsh:Infectious and parasitic diseases 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology Parasite hosting Streptomyces hygroscopicus subsp. hygroscopicus lcsh:RC109-216 0303 health sciences biology 030306 microbiology Chemistry Inoculation Plasmodium falciparum biology.organism_classification Molecular biology In vitro Infectious Diseases Plasmodium falciparum 3D7 Parasitology Fermentation DNA parasite density Streptomyces hygroscopicus |
| Zdroj: | Iranian Journal of Parasitology, Vol 14, Iss 3 (2019) |
| ISSN: | 1735-7020 |
| DOI: | 10.18502/ijpa.v14i3.1484 |
| Popis: | Background: Malaria eradication has been complicated by the repeated emergence of antimalarial drug resistances. We aimed to determine whether a metabolite extract of Streptomyces hygrocopicus subsp. hygroscopicus could decrease the viability of Plasmodium falciparum 3D7 in vitro. Methods: S. hygroscopicus subsp. hygroscopicus isolates were inoculated and fermented on the ISP4 medium. The fermented S. hygroscopicus was mixed with ethylacetate 1:5 (v/v), and the solvent phase was evaporated. Several concentrations of isolated extract was added to the P. falciparum 3D7 culture containing trophozoite and schizont stages in 24 wells plates when the degree of parasite-infected erythrocytes reached 5%, then incubated for 8 hours. DNA parasite density was measured using flow cytometry, parasite degree and morphology were observed under microscopic by Giemsa-stained smears. Results: The metabolite extract affected the morphology of almost all of parasite asexual stages. Schizonts and trophozoites failed to grow and appeared damaged with pycnotic cores and loss of cytoplasmic content. At 8 hours there was a significant decrease in DNA parasite density in culture exposed to 2.6 mg/ml and 13 mg/ml (P = 0.002; P = 0.024) of the extract. The degree of parasite-infected erythrocytes was decreased from the beginning of exposure (0.02 mg/ml of the extract). There was a significant inverse correlation between the concentration of extract and the degree of parasite-infected erythrocytes as well as the density of DNA parasite (r = -0.772, P = 0.000; r =-0.753; P =0.000). Conclusion: Metabolite extract of S. hygroscopicus subsp. hygroscopicus causes morphological damage, decreases the degree of parasite-infected erythrocytes and the DNA density of P. falciparum 3D7 in vitro. |
| Databáze: | OpenAIRE |
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