Lithium Ions Up-Regulate mRNAs Encoding Dense-Core Vesicle Proteins in Nerve Growth Factor-Differentiated PC12 Cells
| Autor: | Joy A. Umbach, Mara L. Cordeiro, Cameron B. Gundersen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Vesicular Monoamine Transport Proteins
Lithium (medication) Vesicular Acetylcholine Transport Proteins Cellular differentiation Synaptophysin Vesicular Transport Proteins Lithium Biology Vesicular monoamine transporter 1 PC12 Cells Biochemistry Cellular and Molecular Neuroscience chemistry.chemical_compound Vesicular Biogenic Amine Transport Proteins Vesicular acetylcholine transporter Nerve Growth Factor Chromogranins medicine Animals RNA Messenger Oligonucleotide Array Sequence Analysis Neurons Membrane Glycoproteins Secretory Vesicles Neuropeptides Membrane Transport Proteins Proteins Cell Differentiation Rats Up-Regulation Cell biology chemistry Carrier Proteins medicine.drug |
| Zdroj: | Journal of Neurochemistry. 75:2622-2625 |
| ISSN: | 0022-3042 |
| DOI: | 10.1046/j.1471-4159.2000.0752622.x |
| Popis: | We recently reported that lithium ions induced an up-regulation of cysteine string protein (CSP) gene expression in nerve growth factor (NGF)-differentiated PC12 cells but not in undifferentiated cells. Concomitantly, expression of two other proteins of regulated secretory pathways, synaptophysin (SY) and SNAP-25, was unaffected by lithium. To assess further the specificity of this effect of lithium, we used cDNA arrays. Our data indicate that lithium ions increase the level of mRNA for proteins such as secretogranin II and vesicular monoamine transporter 1 that are preferentially associated with large densecore secretory vesicles (LDCVs) without affecting mRNAs for proteins predominantly affiliated with small synaptic-like vesicles, including the vesicular acetylcholine transporter and SY. This action of lithium is detected in NGF-differentiated PC12 cells but not in undifferentiated cells. These observations suggest that lithium ions modulate the turnover of LDCVs, and this may play a role in mediating the therapeutic action of lithium in manic-depressive illness. |
| Databáze: | OpenAIRE |
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