Isolation, identification, and partial cDNA cloning of genomic RNA of jaagsiekte retrovirus, the etiological agent of sheep pulmonary adenomatosis
| Autor: | Robert Vigne, D. W. Verwoerd, Gilles Quérat, D. F. York |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
viruses
Immunology Molecular Sequence Data Centrifugation Isopycnic Microbiology Genome Virus Retrovirus Virology Complementary DNA Animals Amino Acid Sequence Cloning Molecular Lung Cloning Sheep biology Lentivirus RNA Gene Products env DNA Jaagsiekte sheep retrovirus biology.organism_classification Blotting Northern Molecular biology Retroviridae Pulmonary Adenomatosis Ovine Insect Science RNA Viral Oncovirus Research Article |
| Zdroj: | Journal of virology. 65(9) |
| ISSN: | 0022-538X |
| Popis: | The genome of the jaagsiekte (JS) retrovirus (JSRV), the etiological agent of sheep pulmonary adenomatosis (jaagsiekte), has been identified, isolated, and partly cloned. The JSRV genome is ca. 8.7 kb long. cDNA of the genomic RNA was synthesized and cloned. A clone, JS 46.1, was isolated and characterized. It has an insert of 2.1 kb which hybridizes to the same 8.7-kb RNA in all the JSRV-infected sheep lung washes tested but does not hybridize to maedi-visna virus, a sheep lentivirus often found coinfecting JSRV-infected lungs. Comparison of the amino acid sequence encoded by JS 46.1 with those encoded by other retroviruses revealed that JSRV has homology to the type D and B oncoviruses and to human endogenous retrovirus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|